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Structure of a Glycosylphosphatidylinositol-anchored Domain from a Trypanosome Variant Surface Glycoprotein
Authors
Jones, N.G., Nietlispach, D., Sharma, R., Burke, D.F., Eyres, I., Mues, M., Mott, H.R., Carrington, M.
Assembly
ILTat1.24 C2 domain
Entity
1. ILTat1.24 C2 domain (polymer, Thiol state: all disulfide bound), 48 monomers, 4919.414 Da Detail

GTKASKSGVP VTQTQTAGAD TTAEKCKGKG EKDCKSPDCK WEGGTCKD


Formula weight
4919.414 Da
Source organism
Trypanosoma brucei
Exptl. method
solution NMR
Refine. method
torsion angle dynamics, simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.9 %, Completeness: 98.5 %, Completeness (bb): 97.0 % Detail

Polymer type: polypeptide(L)

Total1H
All98.5 % (259 of 263)98.5 % (259 of 263)
Backbone97.0 % (98 of 101)97.0 % (98 of 101)
Sidechain99.4 % (161 of 162)99.4 % (161 of 162)
Aromatic100.0 % (6 of 6)100.0 % (6 of 6)
Methyl93.3 % (14 of 15)93.3 % (14 of 15)

1. Variant Surface Glycoprotein ILTat1.24

GTKASKSGVP VTQTQTAGAD TTAEKCKGKG EKDCKSPDCK WEGGTCKD

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0


#NameIsotope labelingTypeConcentration
1ILTat1.24 C2-domainnatural abundance0.5 mM
2sodium phosphatenatural abundance50 mM
3sodium chloridenatural abundance150 mM
4sodium azidenatural abundance0.05 %
5D2O10 %
6H2Onatural abundance90 %

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Calculated from 35 models in PDB: 2JWH, Strand ID: A Detail


Release date
2008-11-11
Citation
Structure of a glycosylphosphatidylinositol-anchored domain from a trypanosome variant surface glycoprotein
Jones, N.G., Nietlispach, D., Sharma, R., Burke, D.F., Eyres, I., Mues, M., Mott, H.R., Carrington, M.
J. Biol. Chem. (2008), 283, 3584-3593, PubMed 18003615 , DOI 10.1074/jbc.M706207200 ,
Related entities 1. ILTat1.24 C2 domain, : 1 : 1 : 13 entities Detail
Experiments performed 1 experiments Detail
NMR combined restraints 3 contents Detail
Keywords Immune System, Membrane Protein, VSG type 1 C-terminal domain