NMR structure of adenosine bulged RNA duplex with C:G-A triple
Polymer type: polyribonucleotide
Total | 1H | 13C | 31P | |
---|---|---|---|---|
All | 46.8 % (131 of 280) | 48.0 % (72 of 150) | 46.0 % (52 of 113) | 41.2 % (7 of 17) |
Suger, PO4 | 45.6 % (93 of 204) | 47.1 % (48 of 102) | 44.7 % (38 of 85) | 41.2 % (7 of 17) |
Nucleobase | 50.0 % (38 of 76) | 50.0 % (24 of 48) | 50.0 % (14 of 28) | |
Aromatic | 46.9 % (30 of 64) | 44.4 % (16 of 36) | 50.0 % (14 of 28) |
1. RNA (5'-R(*GP*UP*CP*GP*AP*GP*CP*UP*G)-3')
GUCGAGCUG2. RNA (5'-R(*CP*AP*GP*CP*CP*GP*AP*C)-3')
CAGCCGACSolvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RNA (5'-R(*GP*UP*CP*GP*AP*GP*CP*UP*G)-3') | natural abundance | 1 mM | |
2 | RNA (5'-R(*CP*AP*GP*CP*CP*GP*AP*C)-3') | natural abundance | 1 mM | |
3 | NaCl | 50 mM | ||
4 | phosphate buffer | 10 mM | ||
5 | EDTA | 0.1 mM | ||
6 | D2O | 100 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | RNA (5'-R(*GP*UP*CP*GP*AP*GP*CP*UP*G)-3') | natural abundance | 1 mM | |
8 | RNA (5'-R(*CP*AP*GP*CP*CP*GP*AP*C)-3') | natural abundance | 1 mM | |
9 | NaCl | 50 mM | ||
10 | phosphate buffer | 10 mM | ||
11 | EDTA | 0.1 mM | ||
12 | H2O | natural abundance | 90 % | |
13 | D2O | 10 % |