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BMRB: 15884

487D

Backbone 1H, 13C, and 15N Chemical Shift Assignments for CTL9 native state at pH 3.8

Authors

Shan, B., Raleigh, D.

Assembly

CTL9

Entity

1. CTL9 (polymer, Thiol state: not present), 92 monomers, 9981.458 Da Detail

AAEELANAKK LKEQLEKLTV TIPAKAGEGG RLFGSITSKQ IAESLQAQHG LKLDKRKIEL ADAIRALGYT NVPVKLHPEV TATLKVHVTE QK

Formula weight

9981.458 Da

Source organism

Geobacillus stearothermophilus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.7 %, Completeness: 56.3 %, Completeness (bb): 90.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	56.3 % (604 of 1073)	46.4 % (260 of 560)	62.4 % (260 of 417)	87.5 % (84 of 96)
Backbone	90.8 % (496 of 546)	83.4 % (156 of 187)	95.2 % (257 of 270)	93.3 % (83 of 89)
Sidechain	30.7 % (188 of 613)	27.6 % (103 of 373)	36.5 % (85 of 233)	0.0 % (0 of 7)
Aromatic	0.0 % (0 of 30)	0.0 % (0 of 15)	0.0 % (0 of 15)
Methyl	20.0 % (26 of 130)	20.0 % (13 of 65)	20.0 % (13 of 65)

1. CTL9

AAEELANAKK LKEQLEKLTV TIPAKAGEGG RLFGSITSKQ IAESLQAQHG LKLDKRKIEL ADAIRALGYT NVPVKLHPEV TATLKVHVTE QK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.2 (±2) K, pH 3.8 (±0.1), Details 20 mM sodium acetate 100 mM sodium chloride pH 3.8

#	Name	Isotope labeling	Concentration
1	CTL9	[U-100% 13C; U-100% 15N]	1 mM
2	H2O	natural abundance	90 %
3	D2O	[U-100% 2H]	10 %
4	sodium acetate	natural abundance	20 mM
5	sodium chloride	natural abundance	100 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.37 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.37 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.37 ppm, Outliers: 1 Detail

Release date

2008-09-18

Citation

The low-pH unfolded state of the C-terminal domain of the ribosomal protein L9 contains significant secondary structure in the absence of denaturant but is no more compact than the low-pH urea unfolded state
Shan, B., Raleigh, D.P.
Biochemistry (2008), 47, 9565-9573, PubMed 18707127 , DOI 10.1021/bi8006862 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 15883 released on 2008-09-18
Title Backbone and 13C and 1H assignments for acid unfolded state and urea unfolded state of CTL9