1H, 15N chemical shifts of the UHM domain of Puf60 in complex with U2AF65(85-112)
MKHHHHHHPM ESTVMVLRNM VDPKDIDDDL EGEVTEECGK FGAVNRVIIY QEKQGEEEDA EIIVKIFVEF SIASETHKAI QALNGRWFAG RKVVAEVYDQ ERFDNSDLSA
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 18.7 % (197 of 1056) | 12.3 % (111 of 904) | 56.6 % (86 of 152) |
Backbone | 41.0 % (178 of 434) | 31.3 % (92 of 294) | 61.4 % (86 of 140) |
Sidechain | 3.1 % (19 of 622) | 3.1 % (19 of 610) | 0.0 % (0 of 12) |
Aromatic | 0.0 % (0 of 88) | 0.0 % (0 of 86) | 0.0 % (0 of 2) |
Methyl | 1.5 % (1 of 66) | 1.5 % (1 of 66) |
1. UHM domain of Puf60
MKHHHHHHPM ESTVMVLRNM VDPKDIDDDL EGEVTEECGK FGAVNRVIIY QEKQGEEEDA EIIVKIFVEF SIASETHKAI QALNGRWFAG RKVVAEVYDQ ERFDNSDLSA2. U2AF65(85-112)
KKKVRKYWDV PPPGFEHITP MQYKAMQAGS HHHHHHSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 295 (±0.1) K, pH 6.8 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | UHM domain of Puf60 | [U-100% 13C; U-100% 15N] | 0.177 (±0.01) mM | |
2 | U2AF65(85-112) | natural abundance | 0.333 (±0.01) mM | |
3 | NaH2PO4 | natural abundance | 50 mM | |
4 | NaCl | natural abundance | 150 mM | |
5 | b-mercaptoethanol | natural abundance | 5 mM | |
6 | H2O | natural abundance | 90 % | |
7 | D2O | [U-100% 2H] | 10 % |