1H, 15N chemical shifts of the UHM domain of Puf60 in complex with SF3b155(194-229)
MKHHHHHHPM ESTVMVLRNM VDPKDIDDDL EGEVTEECGK FGAVNRVIIY QEKQGEEEDA EIIVKIFVEF SIASETHKAI QALNGRWFAG RKVVAEVYDQ ERFDNSDLSA
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 20.4 % (216 of 1058) | 13.6 % (122 of 900) | 59.5 % (94 of 158) |
Backbone | 42.9 % (192 of 448) | 32.6 % (99 of 304) | 64.6 % (93 of 144) |
Sidechain | 3.9 % (24 of 610) | 3.9 % (23 of 596) | 7.1 % (1 of 14) |
Aromatic | 0.0 % (0 of 70) | 0.0 % (0 of 67) | 0.0 % (0 of 3) |
Methyl | 7.4 % (5 of 68) | 7.4 % (5 of 68) |
1. UHM domain of Puf60
MKHHHHHHPM ESTVMVLRNM VDPKDIDDDL EGEVTEECGK FGAVNRVIIY QEKQGEEEDA EIIVKIFVEF SIASETHKAI QALNGRWFAG RKVVAEVYDQ ERFDNSDLSA2. SF3b155(194-229)
GAMGSKRKRR WDQTADQTPG ATPKKLSSWD QAETPGHTPSSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 295 (±0.1) K, pH 6.8 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | UHM domain of Puf60 | [U-100% 13C; U-100% 15N] | 0.0914 (±0.01) mM | |
2 | SF3b155(194-229) | natural abundance | 0.422 (±0.01) mM | |
3 | NaH2PO4 | natural abundance | 50 mM | |
4 | NaCl | natural abundance | 150 mM | |
5 | b-mercaptoethanol | natural abundance | 5 mM | |
6 | H2O | natural abundance | 95 % | |
7 | D2O | [U-100% 2H] | 5 % |