1H, 15N chemical shifts of the UHM domain of Puf60 in complex with SF3b155(317-357)
MKHHHHHHPM ESTVMVLRNM VDPKDIDDDL EGEVTEECGK FGAVNRVIIY QEKQGEEEDA EIIVKIFVEF SIASETHKAI QALNGRWFAG RKVVAEVYDQ ERFDNSDLSA
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 19.9 % (211 of 1060) | 13.0 % (117 of 902) | 59.5 % (94 of 158) |
Backbone | 41.7 % (193 of 463) | 31.3 % (99 of 316) | 63.9 % (94 of 147) |
Sidechain | 3.0 % (18 of 597) | 3.1 % (18 of 586) | 0.0 % (0 of 11) |
Aromatic | 0.0 % (0 of 61) | 0.0 % (0 of 59) | 0.0 % (0 of 2) |
Methyl | 12.7 % (9 of 71) | 12.7 % (9 of 71) |
1. UHM domain of Puf60
MKHHHHHHPM ESTVMVLRNM VDPKDIDDDL EGEVTEECGK FGAVNRVIIY QEKQGEEEDA EIIVKIFVEF SIASETHKAI QALNGRWFAG RKVVAEVYDQ ERFDNSDLSA2. SF3b155(317-357)
GAMGRGGDSI GETPTPGASK RKSRWDETPA SQMGGSTPVL TPGKSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 295 (±0.1) K, pH 6.8 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | UHM domain of Puf60 | [U-100% 13C; U-100% 15N] | 0.145 (±0.01) mM | |
2 | SF3b155(317-357) | natural abundance | 0.227 (±0.01) mM | |
3 | NaH2PO4 | natural abundance | 50 mM | |
4 | NaCl | natural abundance | 150 mM | |
5 | b-mercaptoethanol | natural abundance | 5 mM | |
6 | H2O | natural abundance | 95 % | |
7 | D2O | [U-100% 2H] | 5 % |