BMRBj - BMREntryMaster

Found 1 Document (0.524 seconds)

BMRB: 15904

1BU5

1J coupling constants related to the Ca carbons in oxidised Flavodoxin from Desulfovibrio vulgaris (Hildenborough)

Authors: Schmidt, J.M., Lohr, F.
Assembly: Flavodoxin oxidized
Entity: 1. Flavodoxin (polymer, Thiol state: all free), 147 monomers, 15692.04 Da Detail
PKALIVYGST TGNTEYTAET IARELADAGY EVDSRDAASV EAGGLFEGFD LVLLGCSTWG DDSIELQDDF IPLFDSLEET GAQGRKVACF GCGDSSYEYF CGAVDAIEEK LKNLGAEIVQ DGLRIDGDPR AARDDIVGWA HDVRGAI

2. FMN (non-polymer), 456.344 Da
Total weight: 16148.384 Da
Max. entity weight: 15692.04 Da
Source organism: Desulfovibrio vulgaris
Exptl. method: solution NMR
Data set: coupling_constants
Coupling constant: 535 J values in 4 lists
Field strength (¹H) 500 MHz, 600 MHz, 800 MHz, Pressure 1 atm, Temperature 300 K, pH 7.0 Detail
Pressure 1 atm, Temperature 300 K, pH 7.0
Experiment name 3D Ha-coupled ct-[15N,1H]-TROSY-iHNCA, 3D quantitative J-correlated [15N,1H]-TROSY-HNC, 3D Ca-coupled [15N,1H]-TROSY-HNCO, 3D Ca-coupled HA(CACO)NH, 2D IPAP-type HN(CO-a/b-NCa-J)-TROSY, 3D Ha-coupled ct-[15N,1H]-TROSY-HNCA, 3D Cb-coupled [15N,1H]-TROSY-HN(CO)CA, 3D 1Ha-13Ca multiple-quantum HCAN quantitative J correlation, 3D Ha-coupled ct-[15N,1H]-TROSY-HN(CO)CA
List #1 1JCaCO, Field strength (¹H) 500 MHz
List #2 1JCaN_, Field strength (¹H) 800 MHz
List #3 1JCaHa, Field strength (¹H) 600 MHz, Details 28-ms constant-time Ca evolution in 3D TROSY experiments
List #4 1JCaCb, Field strength (¹H) 800 MHz, Details Ca evolution time typically 70-90ms
Release date: 2008-11-18
Citation 1: Variation in protein C(alpha)-related one-bond J couplings
Schmidt, J.M., Howard, M.J., Maestre-Martinez, M., Perez, C.S., Lohr, F.
Magn. Reson. Chem. (2009), 47, 16-30, PubMed 18853398 , DOI 10.1002/mrc.2337 , Abstract
Citation 2: Sequential backbone assignment of isotopically enriched proteins in D2O by deuterium-decoupled HA(CA)N and HA(CACO)N
Wang, A.C., Grzesiek, S., Tschudin, R., Lodi, P.J., Bax, A.
J. Biomol. NMR (1995), 5, 376-382, PubMed 7647557 , DOI: , Abstract
Citation 3: TROSY in triple-resonance experiments: new perspectives for sequential NMR assignment of large proteins
Salzmann, M., Pervushin, K., Wider, G., Senn, H., Wuthrich, K.
Proc. Natl. Acad. Sci. U. S. A. (1998), 95, 13585-13590, PubMed 9811843 , Abstract
Citation 4: Recording heteronuclear quantitative J-correlation spectra with internal reference peaks
Lohr, F., Perez, C., Schmidt, J.M., Ruterjans, H.
Bull. Magn. Reson. (1999), 20, 9-14
Citation 5: TROSY-type triple-resonance experiments for sequential NMR assignments of large proteins
Salzmann, M., Wider, G., Pervushin, K., Senn, H., Wuthrich, K.
J. Am. Chem. Soc. (1999), 121, 844-848, DOI 10.1021/ja9834226
Citation 6: TROSY-based HNCO pulse sequence for the measurement of 1HN-15N, 15N-13CO, 1HN-13CO, 13CO-13Ca and 1HN-13Ca dipolar couplings in 15N, 13C, 2H-labeled proteins
Yang, D., Venters, R.A., Mueller, G.A., Choy, W.Y., Kay, L.E.
J. Biomol. NMR (1999), 14, 333-343
Citation 7: Transverse relaxation optimised spin-state selective NMR experiments for measurement of residual dipolar couplings
Permi, P., Annila, A.
J. Biomol. NMR (2000), 16, 221-227, PubMed 10805128 , Abstract
Citation 8: Measurement of (13)C(alpha)-(13)C(beta) dipolar couplings in (15)N,(13)C,(2)H-labeled proteins: application to domain orientation in maltose binding protein
Evenas, J., Mittermaier, A., Yang, D., Kay, L.E.
J. Am. Chem. Soc. (2001), 123, 2858-2864, PubMed 11456973 , DOI: , Abstract
Citation 9: Intraresidue HNCA and COHNCA experiments for protein backbone resonance assignment
Brutscher, B.
J. Magn. Reson. (2002), 156, 155-159, PubMed 12081454 , Abstract
Citation 10: A novel approach for the sequential backbone assignment of larger proteins: selective intra-HNCA and DQ-HNCA
Nietlispach, D., Ito, Y., Laue, E.D.
J. Am. Chem. Soc. (2002), 124, 11199-11207, PubMed 12224968 , DOI: , Abstract
Citation 11: Intraresidual HNCA: an experiment for correlating only intraresidual backbone resonances
Permi, P.
J. Biomol. NMR (2002), 23, 201-209, PubMed 12238592 , Abstract
Citation 12: A high sensitivity 3D experiment for measuring Calpha-Halpha residual dipolar coupling constants
Hu, W., Zhang, Z., Chen, Y.
J. Magn. Reson. (2003), 165, 248-252, PubMed 14643706 , DOI 10.1016/j.jmr.2003.08.008 , Abstract
Citation 13: Measurement of residual dipolar couplings from 1Halpha to 13Calpha and 15N using a simple HNCA-based experiment
Permi, P.
J. Biomol. NMR (2003), 27, 341-349, PubMed 14512731 , Abstract
Citation 14: Simultaneous measurement of protein one-bond and two-bond nitrogen-carbon coupling constants using an internally referenced quantitative J-correlated [(15)N,(1)H]-TROSY-HNC experiment
Wienk, H.L.J., Martinez, M.M., Yalloway, G.N., Schmidt, J.M., Perez, C., Ruterjans, H., Lohr, F.
J. Biomol. NMR (2003), 25, 133-145, PubMed 12652122 , Abstract
Citation 15: Measurement of one-bond 13Calpha-1Halpha residual dipolar coupling constants in proteins by selective manipulation of CalphaHalpha spins
Ball, G., Meenan, N., Bromek, K., Smith, B.O., Bella, J., Uhrin, D.
J. Magn. Reson. (2006), 180, 127-136, PubMed 16495100 , DOI 10.1016/j.jmr.2006.01.017 , Abstract

Related entities 1. Flavodoxin, : 1 : 1 : 4 : 19 : 176 entities Detail

Experiments performed 9 experiments Detail

1 3D Ca-coupled [15N,1H]-TROSY-HNCO

Instrument

Bruker DRX - 500 MHz 1H{13C,15N}-triple-resonance three-axis PFG probe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Flavodoxin	[U-98% 13C; U-98% 15N]	2.1 mM
2	potassium phosphate	natural abundance	10 mM
3	D2O	[U-2H]	5 %
4	H2O	natural abundance	95 %

2 3D Cb-coupled [15N,1H]-TROSY-HN(CO)CA

Instrument

Bruker Avance - 800 MHz room-temperature and cryogenic triple-resonance z-gradient probes

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Flavodoxin	[U-98% 13C; U-98% 15N]	2.1 mM
2	potassium phosphate	natural abundance	10 mM
3	D2O	[U-2H]	5 %
4	H2O	natural abundance	95 %

3 2D IPAP-type HN(CO-a/b-NCa-J)-TROSY

Instrument

Bruker Avance - 800 MHz room-temperature and cryogenic triple-resonance z-gradient probes

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Flavodoxin	[U-98% 13C; U-98% 15N]	2.1 mM
2	potassium phosphate	natural abundance	10 mM
3	D2O	[U-2H]	5 %
4	H2O	natural abundance	95 %

4 3D quantitative J-correlated [15N,1H]-TROSY-HNC

Instrument

Bruker Avance - 800 MHz room-temperature and cryogenic triple-resonance z-gradient probes

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Flavodoxin	[U-98% 13C; U-98% 15N]	2.1 mM
2	potassium phosphate	natural abundance	10 mM
3	D2O	[U-2H]	5 %
4	H2O	natural abundance	95 %

5 3D Ca-coupled HA(CACO)NH

Instrument

Bruker DMX - 600 MHz 1H{13C,15N}-triple-resonance three-axis PFG probe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Flavodoxin	[U-98% 13C; U-98% 15N]	2.1 mM
2	potassium phosphate	natural abundance	10 mM
3	D2O	[U-2H]	5 %
4	H2O	natural abundance	95 %

6 3D Ha-coupled ct-[15N,1H]-TROSY-HNCA

Instrument

Bruker DRX - 600 MHz cryogenically cooled triple-resonance z-gradient probe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Flavodoxin	[U-98% 13C; U-98% 15N]	2.1 mM
2	potassium phosphate	natural abundance	10 mM
3	D2O	[U-2H]	5 %
4	H2O	natural abundance	95 %

7 3D Ha-coupled ct-[15N,1H]-TROSY-iHNCA

Instrument

Bruker Avance - 700 MHz 1H{13C,15N}-triple-resonance z-gradient probe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Flavodoxin	[U-98% 13C; U-98% 15N]	2.1 mM
2	potassium phosphate	natural abundance	10 mM
3	D2O	[U-2H]	5 %
4	H2O	natural abundance	95 %

8 3D Ha-coupled ct-[15N,1H]-TROSY-HN(CO)CA

Instrument

Bruker Avance - 800 MHz room-temperature and cryogenic triple-resonance z-gradient probes

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Flavodoxin	[U-98% 13C; U-98% 15N]	2.1 mM
2	potassium phosphate	natural abundance	10 mM
3	D2O	[U-2H]	5 %
4	H2O	natural abundance	95 %

9 3D 1Ha-13Ca multiple-quantum HCAN quantitative J correlation

Instrument

Bruker DRX - 500 MHz 1H{13C,15N}-triple-resonance three-axis PFG probe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Flavodoxin	[U-98% 13C; U-98% 15N]	2.1 mM
2	potassium phosphate	natural abundance	10 mM
3	D2O	[U-2H]	5 %
4	H2O	natural abundance	95 %

Keywords data mining, one-bond coupling, statistics, torsion angles, 1JCAN measurement in proline, 1JCACO measurement, 1JCAHA measurement, TROSY method, 1JCAN measurement, quantitative J-correlation, 1JCACB measurement

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Found 1 Document (0.524 seconds)

BMRB: 15904

Related entities 1. Flavodoxin, : 1 : 1 : 4 : 19 : 176 entities Detail

Experiments performed 9 experiments Detail

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