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BMRB: 15914

2K9A

Backbone 1H, 13C and 15N Chemical Shift Assignments for Binder of Arl2 (BART)

Authors

Bailey, L.K., Campbell, L.J., Evetts, K.A., Littlefield, K., Rajendra, E., Nietlispach, D., Owen, D., Mott, H.R.

Assembly

Binder of Arl2

Entity

1. Binder of Arl2 (polymer, Thiol state: all free), 137 monomers, 16149.88 Da Detail

HMDALEGESF ALSFSSASDA EFDAVVGYLE DIIMDDEFQL LQRNFMDKYY LEFEDTEENK LIYTPIFNEY ISLVEKYIEE QLLQRIPEFN MAAFTTTLQH HKDEVAGDIF DMLLTFTDFL AFKEMFLDYR AEKEGRG

Formula weight

16149.88 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.2 %, Completeness: 89.3 %, Completeness (bb): 89.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.3 % (1479 of 1657)	90.0 % (769 of 854)	87.9 % (579 of 659)	91.0 % (131 of 144)
Backbone	89.1 % (729 of 818)	90.3 % (250 of 277)	87.9 % (357 of 406)	90.4 % (122 of 135)
Sidechain	89.7 % (871 of 971)	89.9 % (519 of 577)	89.1 % (343 of 385)	100.0 % (9 of 9)
Aromatic	80.8 % (168 of 208)	80.8 % (84 of 104)	80.8 % (84 of 104)
Methyl	91.5 % (130 of 142)	91.5 % (65 of 71)	91.5 % (65 of 71)

1. Binder of Arl2

HMDALEGESF ALSFSSASDA EFDAVVGYLE DIIMDDEFQL LQRNFMDKYY LEFEDTEENK LIYTPIFNEY ISLVEKYIEE QLLQRIPEFN MAAFTTTLQH HKDEVAGDIF DMLLTFTDFL AFKEMFLDYR AEKEGRG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 15N]	1 mM
2	D2O	natural abundance	10 %
3	sodium phosphate	natural abundance	50 mM
4	sodium chloride	natural abundance	150 mM
5	sodium azide	natural abundance	0.05 %
6	H2O	natural abundance	90 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Concentration
7	entity	[U-100% 13C; U-100% 15N]	1 mM
8	D2O	natural abundance	10 %
9	sodium phosphate	natural abundance	50 mM
10	sodium chloride	natural abundance	150 mM
11	sodium azide	natural abundance	0.05 %
12	H2O	natural abundance	90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.43 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 50 models in PDB: 2K9A, Strand ID: A Detail

Release date

2008-11-18

Citation

The structure of binder of Arl2 (BART) reveals a novel G protein binding domain: implications for function
Bailey, L.K., Campbell, L.J., Evetts, K.A., Littlefield, K., Rajendra, E., Nietlispach, D., Owen, D., Mott, H.R.
J. Biol. Chem. (2009), 284, 992-999, PubMed 18981177 , DOI 10.1074/jbc.M806167200 , Abstract

Entries sharing articles Swiss-Prot: 2 entries Detail

  Swiss-Prot: Q9Y2Y0 released on 2007-05-15
    Title AR2BP_HUMAN Entity ADP-ribosylation factor-like protein 2-binding protein
  Swiss-Prot: P36404 released on 1994-06-01
    Title ARL2_HUMAN Entity ADP-ribosylation factor-like protein 2

Related entities 1. Binder of Arl2, : 1 : 6 : 15 entities Detail

Interaction partners 1. Binder of Arl2, : 8 interactors Detail

More details for 8 interactors identified by 4 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC