Deletions in a surface loop divert the folding of a protein domain into a metastable dimeric form
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 81.0 % (460 of 568) | 80.2 % (388 of 484) | 85.7 % (72 of 84) |
Backbone | 87.3 % (219 of 251) | 86.5 % (148 of 171) | 88.7 % (71 of 80) |
Sidechain | 76.0 % (241 of 317) | 76.7 % (240 of 313) | 25.0 % (1 of 4) |
Aromatic | 100.0 % (10 of 10) | 100.0 % (10 of 10) | |
Methyl | 88.7 % (55 of 62) | 88.7 % (55 of 62) |
1. E2plipD4
MVKEVNVPDI VEVTEVMVKV GDKVAAEQSL ITVEGDKASM EVPAPFAGVV KELKVNVGDK VKTGSLIMIF EVEGAAPAAA PAKQESolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | E2plipD4 | [U-15N] | 1 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | TSP | natural abundance | 40 uM | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |