BMRB: 15959

Backbone 1H and 15N Chemical Shift Assignments for the Phosphotyrosine Binding Domain of Insulin Receptor Substrate 1 in the Apo and Phosphopeptide Bound Forms

Authors

Stone, M.J., Jarymowycz, V.A.

Assembly

Phosphotyrosine-Binding Domain of Insulin Receptor Substrate-1 Monomer

Entity

1. Phosphotyrosine-Binding Domain of Insulin Receptor Substrate-1 Monomer (polymer, Thiol state: all free), 111 monomers, 12502.42 Da Detail

GPAFKEVWQV ILKPKGLGQT KNLIGIYRLC LTSKTISFVK LNSEAAAVVL QLMNIRRCGH SENFFFIEVG RSAVTGPGEF WMQVDDSVVA QNMHETILEA MRAMSDEFRP R

Formula weight

12502.42 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.6 %, Completeness: 30.2 %, Completeness (bb): 64.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	30.2 % (243 of 804)	19.9 % (136 of 685)	89.9 % (107 of 119)
Backbone	64.9 % (216 of 333)	49.1 % (111 of 226)	98.1 % (105 of 107)
Sidechain	5.7 % (27 of 471)	5.4 % (25 of 459)	16.7 % (2 of 12)
Aromatic	14.0 % (8 of 57)	10.9 % (6 of 55)	100.0 % (2 of 2)
Methyl	3.1 % (2 of 65)	3.1 % (2 of 65)

1. Phosphotyrosine-Binding Domain of Insulin Receptor Substrate-1

GPAFKEVWQV ILKPKGLGQT KNLIGIYRLC LTSKTISFVK LNSEAAAVVL QLMNIRRCGH SENFFFIEVG RSAVTGPGEF WMQVDDSVVA QNMHETILEA MRAMSDEFRP R

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Phosphotyrosine-Binding Domain of Insulin Receptor Substrate-1	[U-95% 13C]		200 uM

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Release date

2008-10-02

Citation

Remote changes in the dynamics of the phosphotyrosine-binding domain of insulin receptor substrate-1 induced by phosphopeptide binding
Stone, M.J., Jarymowycz, V.A.
Biochemistry (2008), 47, 13371-13382, PubMed 19053277 , DOI 10.1021/bi801096b , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Phosphotyrosine-Binding Domain of Insulin Receptor Substrate-1 Monomer, : 1 : 8 : 1 : 34 entities Detail

Interaction partners 1. Phosphotyrosine-Binding Domain of Insulin Receptor Substrate-1 Monomer, : 19 interactors Detail

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail