Structure of TM1_TM2 in LPPG micelles
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 89.0 % (837 of 940) | 86.1 % (408 of 474) | 92.6 % (351 of 379) | 89.7 % (78 of 87) |
Backbone | 96.7 % (462 of 478) | 97.6 % (160 of 164) | 96.2 % (226 of 235) | 96.2 % (76 of 79) |
Sidechain | 83.8 % (450 of 537) | 80.0 % (248 of 310) | 91.3 % (200 of 219) | 25.0 % (2 of 8) |
Aromatic | 57.8 % (52 of 90) | 57.8 % (26 of 45) | 56.8 % (25 of 44) | 100.0 % (1 of 1) |
Methyl | 98.5 % (132 of 134) | 97.0 % (65 of 67) | 100.0 % (67 of 67) |
1. TM1 TM2 in LPPG micelles
GNGSTITFDE LQGLVNSTVT QAILFGVRSG AAALTLIVVW ITSRSRKTPI FIINQVSLFL IILHSALYFK YLLSNYSSVTSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 320 K, pH 6.4, Details for backbone assignment
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | LPPG | natural abundance | 200 mM | |
2 | Na-phosphate buffer | natural abundance | 20 mM | |
3 | TM1-TM2 peptide | [U-100% 13C; U-100% 15N] | 0.4 mM | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 320 K, pH 6.4, Details for 15N-NOESY measurement
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | LPPG | natural abundance | 200 mM | |
7 | Na-phosphate buffer | natural abundance | 20 mM | |
8 | TM1-TM2 peptide | [U-100% 15N] | 0.4 mM | |
9 | H2O | natural abundance | 90 % | |
10 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 320 K, pH 6.4, Details for 15N-NOESY measurement on a 80%-deuterated background
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | LPPG | natural abundance | 200 mM | |
12 | Na-phosphate buffer | natural abundance | 20 mM | |
13 | TM1-TM2 peptide | [U-100% 15N; 80% 2H] | 0.4 mM | |
14 | H2O | natural abundance | 90 % | |
15 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 320 K, pH 6.4, Details for 13C-NOESY measurement
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
16 | LPPG | [>85% 2H] | 200 mM | |
17 | Na-phosphate buffer | natural abundance | 20 mM | |
18 | TM1-TM2 peptide | [U-100% 13C; U-100% 15N] | 0.4 mM | |
19 | H2O | natural abundance | 90 % | |
20 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 320 K, pH 6.4, Details For 13C-NOESY measurement on selectively protonated Me-groups of Ile, Leu and Val residues on otherwise deuterated background.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
21 | LPPG | [>85% 2H] | 200 mM | |
22 | Na-phosphate buffer | natural abundance | 20 mM | |
23 | TM1-TM2 peptide | [U-100% 13C; U-100% 15N; >98% 2H; Me(Ile(Hd1),Leu(Hd),Val(Hg)) 1H] | 0.4 mM | |
24 | D2O | natural abundance | 100 % |