NMR Structure of the protein TM1081
MGSDKIHHHH HHMFPYKIVD DVVILMPNKE LNIENAHLFK KWVFDEFLNK GYNKIFLVLS DVESIDSFSL GVIVNILKSI SSSGGFFALV SPNEKVERVL SLTNLDRIVK IYDTISEAME EVRRK
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 80.3 % (1226 of 1526) | 87.6 % (692 of 790) | 68.9 % (417 of 605) | 89.3 % (117 of 131) |
Backbone | 76.5 % (569 of 744) | 89.7 % (226 of 252) | 63.2 % (234 of 370) | 89.3 % (109 of 122) |
Sidechain | 85.0 % (767 of 902) | 86.6 % (466 of 538) | 82.5 % (293 of 355) | 88.9 % (8 of 9) |
Aromatic | 56.3 % (81 of 144) | 68.1 % (49 of 72) | 45.1 % (32 of 71) | 0.0 % (0 of 1) |
Methyl | 99.4 % (157 of 158) | 98.7 % (78 of 79) | 100.0 % (79 of 79) |
1. TM1081
MGSDKIHHHH HHMFPYKIVD DVVILMPNKE LNIENAHLFK KWVFDEFLNK GYNKIFLVLS DVESIDSFSL GVIVNILKSI SSSGGFFALV SPNEKVERVL SLTNLDRIVK IYDTISEAME EVRRKSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TM1081 | [U-98% 13C; U-98% 15N] | 1.3 mM | |
2 | sodium azide | natural abundance | 0.3 % | |
3 | sodium chloride | natural abundance | 150 mM | |
4 | sodium phosphate | natural abundance | 20 mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |