BMRBj - BMREntryMaster

Found 1 Document (0.74 seconds)

BMRB: 16026

8ONP

ENHANCING THE ACTIVITY OF INSULIN BY STEREOSPECIFIC UNFOLDING

Authors

Hua, Q.X., Xu, B., Huang, K., Hu, S.Q., Nakarawa, S., Jia, W.H., Philips, N.F.PHIL., Wittaker, L., Wittaker, J., Katsoyannis, P.G., Weiss, M.A.

Assembly

INSULIN A CHAIN, INSULIN B CHAIN

Entity

1. INSULIN A CHAIN (polymer, Thiol state: all disulfide bound), 21 monomers, 2383.696 Da Detail

GIVEQCCTSI CSLYQLENYC N

2. INSULIN B CHAIN (polymer, Thiol state: all disulfide bound), 30 monomers, 3331.772 Da Detail

FVNQHLCGSD LVEALYLVCG ERGXFYTKPT

Total weight

5715.468 Da

Max. entity weight

3331.772 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.1 %, Completeness: 94.8 %, Completeness (bb): 88.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	94.8 % (276 of 291)	94.8 % (276 of 291)
Backbone	88.3 % (91 of 103)	88.3 % (91 of 103)
Sidechain	98.4 % (185 of 188)	98.4 % (185 of 188)
Aromatic	96.4 % (27 of 28)	96.4 % (27 of 28)
Methyl	100.0 % (28 of 28)	100.0 % (28 of 28)

1. INSULIN A CHAIN

GIVEQCCTSI CSLYQLENYC N

2. INSULIN B CHAIN

FVNQHLCGSD LVEALYLVCG ERGXFYTKPT

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5-0.8 MM PROTEIN, 90% H2O/ 10% D2O AND 20% ACETIC ACID

#	Name	Isotope labeling	Concentration
1	INSULIN A CHAIN	natural abundance	0.5-0.8 mM
2	INSULIN B CHAIN	natural abundance	0.5-0.8 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %
5	ACETIC ACID	natural abundance	20 %

Release date

2009-04-16

Citation

Enhancing the activity of a protein by stereospecific unfolding: conformational life cycle of insulin and its evolutionary origins
Hua, Q.X., Xu, B., Huang, K., Hu, S.Q., Nakarawa, S., Jia, W., Wang, S.H., Wittaker, J., Katsoyannis, P.G., Weiss, M.A.
J. Biol. Chem. (2009), 284, 14586-14596, PubMed 19321436 , DOI 10.1074/jbc.M900085200 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 16027 released on 2009-04-16
Title ENHANCING THE ACTIVITY OF INSULIN BY STEREOSPECIFIC UNFOLDING

Related entities 1. INSULIN A CHAIN, : 1 : 111 : 13 : 7 : 90 entities Detail

Related entities 2. INSULIN B CHAIN, : 1 : 118 : 96 entities Detail

Interaction partners 1. INSULIN A CHAIN, : 1 : 1 interactors Detail

More details for 2 interactors identified by 2 citations

Interaction partners 2. INSULIN B CHAIN, : 6 : 1 interactors Detail

More details for 7 interactors identified by 7 citations

Experiments performed 3 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr16026_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

Chain_ID	Seq_ID	Comp_ID	Chem_comp_name	Experimental evidences
2	24	DAL	D-ALANINE	Assigned chemical shifts

Content subtype: bmr16026_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	337		95.2 (chain: 1, length: 21), 100.0 (chain: 2, length: 30)
1	Chemical shift references	chemical_shift_reference_1	OK	1		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 21, Sequence coverage: 95.2%
  - Entity_assembly_ID: 2, Chain length: 30, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 337
- Completeness of assignments
  - Entity_assemble_ID: 1
  - Entity_assemble_ID: 2
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
  - Chain_ID: 2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	120	111	92.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	43	35	81.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	77	76	98.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	11	11	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	8	8	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	174	168	96.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	62	58	93.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	112	110	98.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	18	18	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	20	19	95.0

Keywords HORMONE, INSULIN, MUTANT

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Found 1 Document (0.74 seconds)

BMRB: 16026

Sample

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. INSULIN A CHAIN, : 1 : 111 : 13 : 7 : 90 entities Detail

Related entities 2. INSULIN B CHAIN, : 1 : 118 : 96 entities Detail

Interaction partners 1. INSULIN A CHAIN, : 1 : 1 interactors Detail

Interaction partners 2. INSULIN B CHAIN, : 6 : 1 interactors Detail

Experiments performed 3 experiments Detail

Instrument

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Instrument

Sample

Instrument

Sample

Chemical shift validation 4 contents Detail