Solution NMR structure of ubiquitin-like domain of Arabidopsis thaliana protein At2g32350. Northeast Structural Genomics Consortium target AR3433A
MGHHHHHHSH AAVRKIHVTV KFPSKQFTVE VDRTETVSSL KDKIHIVENT PIKRMQLYYS GIELADDYRN LNEYGITEFS EIVVFLKSIN RAKD
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 87.5 % (990 of 1131) | 88.4 % (518 of 586) | 85.9 % (384 of 447) | 89.8 % (88 of 98) |
Backbone | 89.5 % (501 of 560) | 88.4 % (167 of 189) | 90.3 % (252 of 279) | 89.1 % (82 of 92) |
Sidechain | 86.4 % (572 of 662) | 88.4 % (351 of 397) | 83.0 % (215 of 259) | 100.0 % (6 of 6) |
Aromatic | 51.9 % (56 of 108) | 64.8 % (35 of 54) | 38.9 % (21 of 54) | |
Methyl | 99.1 % (107 of 108) | 100.0 % (54 of 54) | 98.1 % (53 of 54) |
1. ar3433a protein
MGHHHHHHSH AAVRKIHVTV KFPSKQFTVE VDRTETVSSL KDKIHIVENT PIKRMQLYYS GIELADDYRN LNEYGITEFS EIVVFLKSIN RAKDSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | ar3433a protein | [U-100% 13C; U-100% 15N] | 1.09 mM | |
2 | DSS | natural abundance | 50 uM | |
3 | sodium chloride | natural abundance | 200 mM | |
4 | MES | natural abundance | 20 mM | |
5 | calcium cloride | natural abundance | 5 mM | |
6 | DTT | natural abundance | 10 mM | |
7 | sodium azide | natural abundance | 0.02 % | |
8 | protease inhibitors | natural abundance | 1 na | |
9 | H2O | natural abundance | 90 % | |
10 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | ar3433a protein | [U-5% 13C; U-100% 15N] | 1.15 mM | |
12 | DSS | natural abundance | 50 uM | |
13 | sodium chloride | natural abundance | 200 mM | |
14 | MES | natural abundance | 20 mM | |
15 | calcium cloride | natural abundance | 5 mM | |
16 | DTT | natural abundance | 10 mM | |
17 | sodium azide | natural abundance | 0.02 % | |
18 | protease inhibitors | natural abundance | 1 na | |
19 | H2O | natural abundance | 90 % | |
20 | D2O | natural abundance | 10 % |