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BMRB: 16031

4JQ0

Nav1.5 C-terminal EF-Hand Domain

Authors

Miloushev, V.Z., Levine, J.A., Arbing, M.A., Hunt, J.F., Pitt, G.S., Palmer, A.G.

Assembly

Nav1.5

Entity

1. Nav1.5 (polymer, Thiol state: all free), 129 monomers, 14645.24 Da Detail

MGSSHHHHHH SSGLVPRGSH MASENFSVAT EESTEPLSED DFDMFYEIWE KFDPEATQFI EYSVLSDFAD ALSEPLRIAK PNQISLINMD LPMVSGDRIH CMDILFAFTK RVLGESGEMD ALKIQMEEK

Formula weight

14645.24 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 82.9 %, Completeness: 50.5 %, Completeness (bb): 77.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	50.5 % (750 of 1485)	44.1 % (339 of 769)	53.1 % (311 of 586)	76.9 % (100 of 130)
Backbone	77.4 % (590 of 762)	72.9 % (188 of 258)	79.3 % (302 of 381)	81.3 % (100 of 123)
Sidechain	30.3 % (256 of 846)	29.5 % (151 of 511)	32.0 % (105 of 328)	0.0 % (0 of 7)
Aromatic	3.6 % (5 of 140)	5.7 % (4 of 70)	1.4 % (1 of 69)	0.0 % (0 of 1)
Methyl	17.2 % (20 of 116)	17.2 % (10 of 58)	17.2 % (10 of 58)

1. Nav1.5

MGSSHHHHHH SSGLVPRGSH MASENFSVAT EESTEPLSED DFDMFYEIWE KFDPEATQFI EYSVLSDFAD ALSEPLRIAK PNQISLINMD LPMVSGDRIH CMDILFAFTK RVLGESGEMD ALKIQMEEK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Concentration
1	Nav1.5	[U-98% 13C; U-98% 15N]	0.5 (±0.05) mM
2	TRIS	[D-11 98 %]	20 (±0.2) mM
3	glycine	[D-5 98 %]	100 (±1.0) mM
4	EDTA	[D-16 98 %]	0.1 (±0.01) mM
5	D2O	natural abundance	10 (±0.5) %
6	H2O	natural abundance	90 (±0.5) %
7	NaN3	natural abundance	0.02 (±0.002) %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.21 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.21 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.31 ppm, Outliers: 1 Detail

Release date

2009-01-19

Citation

Solution structure of the NaV1.2 C-terminal EF-hand domain
Miloushev, V.Z., Levine, J.A., Arbing, M.A., Hunt, J.F., Pitt, G.S., Palmer, A.G.
J. Biol. Chem. (2009), 284, 6446-6454, PubMed 19129176 , DOI 10.1074/jbc.M807401200 , Abstract

Related entities 1. Nav1.5, : 1 : 12 : 101 entities Detail

Interaction partners 1. Nav1.5, : 13 interactors Detail

More details for 13 interactors identified by 9 citations

Experiments performed 6 experiments Detail

1 3D HNCO