BlrP1 BLUF
GEFMLTTLIY RSQVHPDRPP VDLDALVHRA SSKNLPLGIT GILLFNGLQF FQVLEGTEEA LESLFSEIQS DPRHRDVVEL MRDYSAYRRF HGTGMRILDL RLFETDGALE EILRFSTFGV TEPVNDRMFR LLSAFIADGG RYCLPEPL
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 96.4 % (1684 of 1747) | 96.7 % (876 of 906) | 95.5 % (663 of 694) | 98.6 % (145 of 147) |
Backbone | 96.7 % (843 of 872) | 96.7 % (289 of 299) | 96.1 % (416 of 433) | 98.6 % (138 of 140) |
Sidechain | 96.4 % (976 of 1012) | 96.7 % (587 of 607) | 96.0 % (382 of 398) | 100.0 % (7 of 7) |
Aromatic | 87.3 % (138 of 158) | 89.9 % (71 of 79) | 84.8 % (67 of 79) | |
Methyl | 98.9 % (176 of 178) | 98.9 % (88 of 89) | 98.9 % (88 of 89) |
1. BlrP1 BLUF
GEFMLTTLIY RSQVHPDRPP VDLDALVHRA SSKNLPLGIT GILLFNGLQF FQVLEGTEEA LESLFSEIQS DPRHRDVVEL MRDYSAYRRF HGTGMRILDL RLFETDGALE EILRFSTFGV TEPVNDRMFR LLSAFIADGG RYCLPEPLSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | BlrP1 BLUF | [U-99% 13C; U-99% 15N] | 0.1-0.7 mM | |
2 | FLAVIN MONONUCLEOTIDE | [U-99% 13C; U-99% 15N] | 0.2-0.7 mM | |
3 | Tris | natural abundance | 50 mM | |
4 | NaCl | natural abundance | 30 mM |
Varian INOVA - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | BlrP1 BLUF | [U-99% 13C; U-99% 15N] | 0.1-0.7 mM | |
2 | FLAVIN MONONUCLEOTIDE | [U-99% 13C; U-99% 15N] | 0.2-0.7 mM | |
3 | Tris | natural abundance | 50 mM | |
4 | NaCl | natural abundance | 30 mM |
Varian INOVA - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | BlrP1 BLUF | [U-99% 13C; U-99% 15N] | 0.1-0.7 mM | |
2 | FLAVIN MONONUCLEOTIDE | [U-99% 13C; U-99% 15N] | 0.2-0.7 mM | |
3 | Tris | natural abundance | 50 mM | |
4 | NaCl | natural abundance | 30 mM |
Varian INOVA - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | BlrP1 BLUF | [U-99% 13C; U-99% 15N] | 0.1-0.7 mM | |
2 | FLAVIN MONONUCLEOTIDE | [U-99% 13C; U-99% 15N] | 0.2-0.7 mM | |
3 | Tris | natural abundance | 50 mM | |
4 | NaCl | natural abundance | 30 mM |
Varian INOVA - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | BlrP1 BLUF | [U-99% 13C; U-99% 15N] | 0.1-0.7 mM | |
2 | FLAVIN MONONUCLEOTIDE | [U-99% 13C; U-99% 15N] | 0.2-0.7 mM | |
3 | Tris | natural abundance | 50 mM | |
4 | NaCl | natural abundance | 30 mM |
Varian INOVA - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | BlrP1 BLUF | [U-99% 13C; U-99% 15N] | 0.1-0.7 mM | |
2 | FLAVIN MONONUCLEOTIDE | [U-99% 13C; U-99% 15N] | 0.2-0.7 mM | |
3 | Tris | natural abundance | 50 mM | |
4 | NaCl | natural abundance | 30 mM |
Varian INOVA - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | BlrP1 BLUF | [U-99% 13C; U-99% 15N] | 0.1-0.7 mM | |
2 | FLAVIN MONONUCLEOTIDE | [U-99% 13C; U-99% 15N] | 0.2-0.7 mM | |
3 | Tris | natural abundance | 50 mM | |
4 | NaCl | natural abundance | 30 mM |
Varian INOVA - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | BlrP1 BLUF | [U-99% 13C; U-99% 15N] | 0.1-0.7 mM | |
2 | FLAVIN MONONUCLEOTIDE | [U-99% 13C; U-99% 15N] | 0.2-0.7 mM | |
3 | Tris | natural abundance | 50 mM | |
4 | NaCl | natural abundance | 30 mM |
Varian INOVA - 800 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | BlrP1 BLUF | [U-99% 13C; U-99% 15N] | 0.1-0.7 mM | |
2 | FLAVIN MONONUCLEOTIDE | [U-99% 13C; U-99% 15N] | 0.2-0.7 mM | |
3 | Tris | natural abundance | 50 mM | |
4 | NaCl | natural abundance | 30 mM |
Varian INOVA - 800 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | BlrP1 BLUF | [U-99% 13C; U-99% 15N] | 0.1-0.7 mM | |
2 | FLAVIN MONONUCLEOTIDE | [U-99% 13C; U-99% 15N] | 0.2-0.7 mM | |
3 | Tris | natural abundance | 50 mM | |
4 | NaCl | natural abundance | 30 mM |
Properties
Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints | combined_16037_2kb2.nef |
Input source #2: Coordindates | 2kb2.cif |
Diamagnetism of the molecular assembly | True (excluding Oxygen atoms) |
Whether the assembly has a disulfide bond | None |
Whether the assembly has a other bond | None |
Whether the assembly contains a cyclic polymer | None |
Overall data processing status | Warning |
Disulfide bonds
NoneOther bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)
NoneNon-standard residues
Chain_ID | Seq_ID | Comp_ID | Chem_comp_name | Experimental evidences |
---|---|---|---|---|
B | 1 | FMN | FLAVIN MONONUCLEOTIDE | Distance restraints |
Sequence alignments
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 GEFMLTTLIYRSQVHPDRPPVDLDALVHRASSKNLPLGITGILLFNGLQFFQVLEGTEEALESLFSEIQSDPRHRDVVELMRDYSAYRRFHGTGMRILDL |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| GEFMLTTLIYRSQVHPDRPPVDLDALVHRASSKNLPLGITGILLFNGLQFFQVLEGTEEALESLFSEIQSDPRHRDVVELMRDYSAYRRFHGTGMRILDL -------110-------120-------130-------140-------- RLFETDGALEEILRFSTFGVTEPVNDRMFRLLSAFIADGGRYCLPEPL |||||||||||||||||||||||||||||||||||||||||||||||| RLFETDGALEEILRFSTFGVTEPVNDRMFRLLSAFIADGGRYCLPEPL
Chain assignments
Entity_assembly_ID (NMR) | Auth_asym_ID (model) | Length | Unmapped | Conflict | Sequence coverage (%) |
---|---|---|---|---|---|
A | A | 148 | 0 | 0 | 100.0 |
Content subtype: combined_16037_2kb2.nef
Assigned chemical shifts
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 GEFMLTTLIYRSQVHPDRPPVDLDALVHRASSKNLPLGITGILLFNGLQFFQVLEGTEEALESLFSEIQSDPRHRDVVELMRDYSAYRRFHGTGMRILDL ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| .EFMLTTLIYRSQVHPDRPPVDLDALVHRASSKNLPLGITGILLFNGLQFFQVLEGTEEALESLFSEIQSDPRHRDVVELMRDYSAYRRFHGTGMRILDL -------110-------120-------130-------140-------- RLFETDGALEEILRFSTFGVTEPVNDRMFRLLSAFIADGGRYCLPEPL |||||||||||||||||||||||||||||||||||||||||||||||| RLFETDGALEEILRFSTFGVTEPVNDRMFRLLSAFIADGGRYCLPEPL
Comp_index_ID | Comp_ID | Atom_ID | CS value (ppm) |
---|---|---|---|
13 | GLN | CD | 180.456 |
15 | HIS | HE2 | 8.248 |
46 | ASN | CG | 175.563 |
49 | GLN | CD | 179.829 |
69 | GLN | CD | 179.217 |
74 | HIS | HE2 | 11.06 |
74 | HIS | NE2 | 164.263 |
125 | ASN | CG | 176.747 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 906 | 888 | 98.0 |
13C chemical shifts | 694 | 665 | 95.8 |
15N chemical shifts | 161 | 152 | 94.4 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 299 | 294 | 98.3 |
13C chemical shifts | 296 | 281 | 94.9 |
15N chemical shifts | 140 | 137 | 97.9 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 607 | 594 | 97.9 |
13C chemical shifts | 398 | 384 | 96.5 |
15N chemical shifts | 21 | 15 | 71.4 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 93 | 92 | 98.9 |
13C chemical shifts | 93 | 92 | 98.9 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 79 | 75 | 94.9 |
13C chemical shifts | 79 | 70 | 88.6 |
Distance restraints
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 GEFMLTTLIYRSQVHPDRPPVDLDALVHRASSKNLPLGITGILLFNGLQFFQVLEGTEEALESLFSEIQSDPRHRDVVELMRDYSAYRRFHGTGMRILDL ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| .EFMLTTLIYRSQVHPDRPPVDLDALVHRASSKNLPLGITGILLFNGLQFFQVLEGTEEALESLFSEIQSDPRHRDVVELMRDYSAYRRFHGTGMRILDL -------110-------120-------130-------140-------- RLFETDGALEEILRFSTFGVTEPVNDRMFRLLSAFIADGGRYCLPEPL |||||||||||||||||||||||||||||||||||||||||||||||| RLFETDGALEEILRFSTFGVTEPVNDRMFRLLSAFIADGGRYCLPEPL
- X | X
Dihedral angle restraints
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 GEFMLTTLIYRSQVHPDRPPVDLDALVHRASSKNLPLGITGILLFNGLQFFQVLEGTEEALESLFSEIQSDPRHRDVVELMRDYSAYRRFHGTGMRILDL |||||||||||| |||||||||||||||||||||||||||| ||||||||||||||||||||||||| ||||||||| |||| |||||| ....LTTLIYRSQVHP..PPVDLDALVHRASSKNLPLGITGILLFN.LQFFQVLEGTEEALESLFSEIQSDP...DVVELMRDY.AYRR.....MRILDL --------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 -------110-------120-------130-------140-------- RLFETDGALEEILRFSTFGVTEPVNDRMFRLLSAFIADGGRYCLPEPL ||||||||||||||| ||| |||||||||||||| RLFETDGALEEILRF......EPV.DRMFRLLSAFIADG -------110-------120-------130---------