BMRBj - BMREntryMaster

Found 1 Document (0.758 seconds)

BMRB: 16053

2KBT

Attachment of an NMR-invisible solubility enhancement tag (INSET) using a sortase-mediated protein ligation method

Authors

Kumeta, H., Kobashigawa, Y., Ogura, K., Inagaki, F.

Assembly

PROTEIN

Entity

1. PROTEIN (polymer, Thiol state: all free), 141 monomers, 15865.17 Da Detail

GPGTFGTAKA RYDFCARDRS ELSLKEGDII KILNKKGQQG WWRGEIYGRI GWFPSNYVEE DYSEYLPETG GGSGSSMEYK LILNGKTLKG ETTTEAVDAA TAEKVFKQYA NDGVDGEWTY DDATKTFTVT EHSLEHHHHH H

Formula weight

15865.17 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing, distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 56.0 %, Completeness: 54.0 %, Completeness (bb): 54.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	54.0 % (870 of 1610)	54.9 % (456 of 831)	52.5 % (331 of 630)	55.7 % (83 of 149)
Backbone	54.3 % (456 of 840)	54.1 % (160 of 296)	54.2 % (220 of 406)	55.1 % (76 of 138)
Sidechain	54.0 % (483 of 894)	55.3 % (296 of 535)	51.7 % (180 of 348)	63.6 % (7 of 11)
Aromatic	47.4 % (90 of 190)	55.8 % (53 of 95)	37.4 % (34 of 91)	75.0 % (3 of 4)
Methyl	50.0 % (60 of 120)	50.0 % (30 of 60)	50.0 % (30 of 60)

1. entity

GPGTFGTAKA RYDFCARDRS ELSLKEGDII KILNKKGQQG WWRGEIYGRI GWFPSNYVEE DYSEYLPETG GGSGSSMEYK LILNGKTLKG ETTTEAVDAA TAEKVFKQYA NDGVDGEWTY DDATKTFTVT EHSLEHHHHH H

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity	[U-13C; U-15N]
2	MES	natural abundance	20 mM
3	DTT	natural abundance	2 mM
4	NaCl	natural abundance	150 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.21 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.21 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.48 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KBT, Strand ID: A Detail

Release date

2009-03-19

Citation

Attachment of an NMR-invisible solubility enhancement tag using a sortase-mediated protein ligation method
Kobashigawa, Y., Kumeta, H., Ogura, K., Inagaki, F.
J. Biomol. NMR (2009), 43, 145-150, PubMed 19140010 , DOI 10.1007/s10858-008-9296-5 , Abstract

Related entities 1. PROTEIN, : 1 : 4 entities Detail

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC