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BMRB: 16071

7TD6

Mouse prion protein fragment 90-231 at pH 7.0

Authors

Damberger, F.F., Wuthrich, K., Hornemann, S., von Schroetter, C.

Assembly

mPrP90

Entity

1. mPrP90 (polymer, Thiol state: all disulfide bound), 144 monomers, 16342.99 Da Detail

GSGQGGGTHN QWNKPSKPKT NLKHVAGAAA AGAVVGGLGG YMLGSAMSRP MIHFGNDWED RYYRENMYRY PNQVYYRPVD QYSNQNNFVH DCVNITIKQH TVTTTTKGEN FTETDVKMME RVVEQMCVTQ YQKESQAYYD GRRS

Formula weight

16342.99 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS92:SG	1:CYS127:SG

Source organism

Mus musculus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.5 %, Completeness: 45.3 %, Completeness (bb): 77.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	45.3 % (750 of 1656)	23.6 % (204 of 864)	66.2 % (417 of 630)	79.6 % (129 of 162)
Backbone	77.9 % (665 of 854)	48.3 % (144 of 298)	94.2 % (393 of 417)	92.1 % (128 of 139)
Sidechain	21.9 % (204 of 931)	10.6 % (60 of 566)	41.8 % (143 of 342)	4.3 % (1 of 23)
Aromatic	3.7 % (6 of 162)	4.9 % (4 of 81)	2.5 % (2 of 79)	0.0 % (0 of 2)
Methyl	26.4 % (29 of 110)	21.8 % (12 of 55)	30.9 % (17 of 55)

1. mPrP90

GSGQGGGTHN QWNKPSKPKT NLKHVAGAAA AGAVVGGLGG YMLGSAMSRP MIHFGNDWED RYYRENMYRY PNQVYYRPVD QYSNQNNFVH DCVNITIKQH TVTTTTKGEN FTETDVKMME RVVEQMCVTQ YQKESQAYYD GRRS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.2 (±0.2) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	mPrP90	[U-99% 13C; U-99% 15N]	1.2 (±0.05) mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	Sodium Phosphate buffer at pH 7.0	natural abundance	5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.39 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.39 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.21 ppm, Outliers: 3 Detail

Release date

2008-12-18

Citation

Prion protein-detergent micelle interactions studied by NMR in solution
Hornemann, S., von Schroetter, C., Damberger, F.F., Wuthrich, K.
J. Biol. Chem. (2009), 284, 22713-22721, PubMed 19546219 , DOI 10.1074/jbc.M109.000430 , Abstract

Related entities 1. mPrP90, : 1 : 4 : 95 entities Detail

Interaction partners 1. mPrP90, : 36 interactors Detail

More details for 36 interactors identified by 12 citations

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC