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1H, 13C, 15N Chemical Shift Assignment for Saccharomyces cerevisiae Acyl Carrier Protein.
Authors
Perez, D.R.
Assembly
ACP single polypeptide chain
Entity
1. ACP single polypeptide chain (polymer, Thiol state: not present), 183 monomers, 19792.24 Da Detail

MGSSHHHHHH ENLYFQSNAE IADEPVKASL LLHVLVAHKL KKSLDSIPMS KTIKDLVGGK STVQNEILGD LGKEFGTTPE KPEETPLEEL AETFQDTFSG ALGKQSSSLL SRLISSKMPG GFTITVARKY LQTRWGLPSG RQDGVLLVAL SNEPAARLGS EADAKAFLDS MAQKYASIVG VDL


Formula weight
19792.24 Da
Source organism
Saccharomyces cerevisiae
Exptl. method
solution NMR
Refine. method
torsion angle dynamics, molecular dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 94.5 %, Completeness: 91.1 %, Completeness (bb): 91.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All91.1 % (1893 of 2079)91.0 % (979 of 1076)90.7 % (740 of 816)93.0 % (174 of 187)
Backbone91.6 % (991 of 1082)91.7 % (342 of 373)90.8 % (485 of 534)93.7 % (164 of 175)
Sidechain90.7 % (1057 of 1165)90.6 % (637 of 703)91.1 % (410 of 450)83.3 % (10 of 12)
Aromatic67.2 % (86 of 128)67.2 % (43 of 64)66.7 % (42 of 63)100.0 % (1 of 1)
Methyl97.7 % (209 of 214)97.2 % (104 of 107)98.1 % (105 of 107)

1. Acyl Carrier Protein

MGSSHHHHHH ENLYFQSNAE IADEPVKASL LLHVLVAHKL KKSLDSIPMS KTIKDLVGGK STVQNEILGD LGKEFGTTPE KPEETPLEEL AETFQDTFSG ALGKQSSSLL SRLISSKMPG GFTITVARKY LQTRWGLPSG RQDGVLLVAL SNEPAARLGS EADAKAFLDS MAQKYASIVG VDL

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5


#NameIsotope labelingTypeConcentration
1Acyl Carrier Protein[U-13C; U-15N]1 mM
2KH2PO4 : K2HPO4 (0.685 : 0.315) buffernatural abundance52 mM

LACS Plot; CA
Referencing offset: 0.1 ppm, Outliers: 4 Detail
LACS Plot; CB
Referencing offset: 0.1 ppm, Outliers: 4 Detail
LACS Plot; HA
Referencing offset: 0.09 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.68 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2ML8, Strand ID: A Detail


Release date
2009-04-15
Citation
1H, 15N, 13C resonance assignment of the acyl carrier protein subunit of the Saccharomyces cerevisiae fatty acid synthase
Perez, D.R., Wider, G.
Biomol. NMR Assign. (2009), 3, 133-136, PubMed 19636964 , DOI 10.1007/s12104-009-9158-4 ,
Related entities 1. ACP single polypeptide chain, : 1 : 13 : 13 entities Detail
Interaction partners 1. ACP single polypeptide chain, : 38 interactors Detail
Experiments performed 5 experiments Detail
NMR combined restraints 3 contents Detail