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Sequence-specific 1H, 15N, and 13C resonance assignments of the 23.7 kDa homodimeric toxin CcdB from Vibrio fischeri.
Authors
Respondek, M., Buts, L., Loris, R., Zangger, K.
Assembly
CcdB homodimer
Entity
1. CcdB homodimer (polymer, Thiol state: all free), 105 monomers, 11868.63 × 2 Da Detail

MSQFTLYKNK DKSSAKTYPY FVDVQSDLLD NLNTRLVIPL TPIELLDKKA PSHLCPTIHI DEGDFIMLTQ QMTSVPVKIL SEPVNELSTF RNEIIAAIDF LITGI


Total weight
23737.26 Da
Max. entity weight
11868.63 Da
Source organism
Aliivibrio fischeri
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 99.0 %, Completeness: 84.7 %, Completeness (bb): 96.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All84.7 % (1066 of 1259)80.0 % (521 of 651)89.2 % (447 of 501)91.6 % (98 of 107)
Backbone96.6 % (595 of 616)94.6 % (194 of 205)98.1 % (307 of 313)95.9 % (94 of 98)
Sidechain76.5 % (571 of 746)73.3 % (327 of 446)82.5 % (240 of 291)44.4 % (4 of 9)
Aromatic43.9 % (36 of 82)43.9 % (18 of 41)43.9 % (18 of 41)
Methyl87.7 % (128 of 146)84.9 % (62 of 73)90.4 % (66 of 73)

1. CcdB

MSQFTLYKNK DKSSAKTYPY FVDVQSDLLD NLNTRLVIPL TPIELLDKKA PSHLCPTIHI DEGDFIMLTQ QMTSVPVKIL SEPVNELSTF RNEIIAAIDF LITGI

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5


#NameIsotope labelingTypeConcentration
1CcdB[U-100% 13C; U-100% 15N]4-6 mg
2sodium phosphatenatural abundance50 mM
3sodium azidenatural abundance0.02 %
4D2Onatural abundance10 %
5H2Onatural abundance90 %

LACS Plot; CA
Referencing offset: 0.16 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: 0.16 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: -0.12 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: 0.32 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2KMT, Strand ID: A, B Detail


Release date
2009-04-13
Citation
Sequence-specific 1H, 15N and 13C resonance assignments of the 23.7-kDa homodimeric toxin CcdB from Vibrio fischeri
Respondek, M., Buts, L., De Jonge, N., Haesaerts, S., Loris, R., Van Melderen, L., Wyns, L., Zangger, K.
Biomol. NMR Assign. (2009), 3, 145-147, PubMed 19636967 , DOI 10.1007/s12104-009-9161-9 ,
Related entities 1. CcdB homodimer, : 1 : 5 : 13 entities Detail
Experiments performed 10 experiments Detail
nullKeywords CcdB, chromosomal ccd operon, homodimeric protein, toxin-antitoxin systems