BMRB: 16159

Beta1A Integrin Cytoplasmic Tail 1H and 15N Chemical Shift Assignments

Authors

Anthis, N.J., Wegener, K.L., Campbell, I.D.

Assembly

beta1A integrin tail

Entity

1. beta1A integrin tail (polymer, Thiol state: not present), 49 monomers, 5709.550 Da Detail

GPKLLMIIHD RREFAKFEKE KMNAKWDTGE NPIYKSAVTT VVNPKYEGK

Formula weight

5709.55 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 98.0 %, Completeness: 83.1 %, Completeness (bb): 96.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	83.1 % (310 of 373)	80.8 % (261 of 323)	98.0 % (49 of 50)
Backbone	96.5 % (139 of 144)	95.9 % (94 of 98)	97.8 % (45 of 46)
Sidechain	74.7 % (171 of 229)	74.2 % (167 of 225)	100.0 % (4 of 4)
Aromatic	29.6 % (8 of 27)	26.9 % (7 of 26)	100.0 % (1 of 1)
Methyl	77.3 % (17 of 22)	77.3 % (17 of 22)

1. beta1A integrin

GPKLLMIIHD RREFAKFEKE KMNAKWDTGE NPIYKSAVTT VVNPKYEGK

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1.0 atm, Temperature 298 K, pH 5.0

#	Name	Isotope labeling	Type	Concentration
1	D2O	[U-2H]		5 %
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		100 mM
4	DTT	natural abundance		1 mM
5	DSS	natural abundance		0.1 mM
6	beta1A integrin	[U-15N]		0.05 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1.0 atm, Temperature 298 K, pH 5.0

#	Name	Isotope labeling	Type	Concentration
7	D2O	[U-2H]		5 %
8	sodium acetate	natural abundance		20 mM
9	sodium azide	natural abundance		0.02 %
10	DSS	natural abundance		0.1 mM
11	beta1A integrin	[U-15N]		1 mM

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Chem. Shift Complete2

Sequence coverage: 91.8 %, Completeness: 55.8 %, Completeness (bb): 80.2 % Detail

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• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
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Polymer type: polypeptide(L)

	Total	1H	15N
All	55.8 % (416 of 746)	49.2 % (318 of 646)	98.0 % (98 of 100)
Backbone	80.2 % (231 of 288)	72.4 % (142 of 196)	96.7 % (89 of 92)
Sidechain	40.2 % (184 of 458)	39.1 % (176 of 450)	100.0 % (8 of 8)
Aromatic	18.5 % (10 of 54)	15.4 % (8 of 52)	100.0 % (2 of 2)
Methyl	38.6 % (17 of 44)	38.6 % (17 of 44)

1. beta1A integrin

GPKLLMIIHD RREFAKFEKE KMNAKWDTGE NPIYKSAVTT VVNPKYEGK

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1.0 atm, Temperature 298 K, pH 6.1

#	Name	Isotope labeling	Type	Concentration
1	D2O	[U-2H]		5 %
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		100 mM
4	DTT	natural abundance		1 mM
5	DSS	natural abundance		0.1 mM
6	beta1A integrin	[U-15N]		0.05 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1.0 atm, Temperature 298 K, pH 6.1

#	Name	Isotope labeling	Type	Concentration
7	D2O	[U-2H]		5 %
8	sodium acetate	natural abundance		20 mM
9	sodium azide	natural abundance		0.02 %
10	DSS	natural abundance		0.1 mM
11	beta1A integrin	[U-15N]		1 mM

Hide sample codition

Release date

2009-10-06

Citation

The structure of an integrin/talin complex reveals the basis of inside-out signal transduction
Anthis, N.J., Wegener, K.L., Ye, F., Kim, C., Goult, B.T., Lowe, E.D., Vakonakis, I., Bate, N., Critchley, D.R., Ginsberg, M.H., Campbell, I.D.
EMBO J. (2009), 28, 3623-3632, PubMed 19798053 , DOI 10.1038/emboj.2009.287 , Abstract

Related entities 1. beta1A integrin tail, : 1 : 15 : 54 entities Detail

Interaction partners 1. beta1A integrin tail, : 27 interactors Detail

Experiments performed 4 experiments Detail

Chemical shift validation 4 contents Detail