BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	95.0 % (1142 of 1202)	95.5 % (590 of 618)	93.6 % (438 of 468)	98.3 % (114 of 116)
Backbone	97.0 % (590 of 608)	98.1 % (205 of 209)	96.0 % (287 of 299)	98.0 % (98 of 100)
Sidechain	92.5 % (637 of 689)	92.7 % (379 of 409)	91.7 % (242 of 264)	100.0 % (16 of 16)
Aromatic	84.5 % (98 of 116)	84.5 % (49 of 58)	82.7 % (43 of 52)	100.0 % (6 of 6)
Methyl	96.7 % (118 of 122)	96.7 % (59 of 61)	96.7 % (59 of 61)

1. F0

GIDPFTMLSS GDLTSASWEL VVRVDHANGE QQTEITLRVS GDLHIGGVML KLVEQMNIAQ DWSDYALWWE QKRCWLLKTH WTLDKCGVQA DANLLFTPQH KM

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	F0	[U-100% 15N]	200 (±5.0) uM
2	DTT	natural abundance	2 (±0.1) mM
3	sodium phosphate	natural abundance	20 (±0.3) mM
4	sodium chloride	natural abundance	50 (±0.3) mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
7	F0	[U-100% 13C; U-100% 15N]	200 (±5.0) uM
8	DTT	natural abundance	2 (±0.1) mM
9	sodium phosphate	natural abundance	20 (±0.3) mM
10	sodium chloride	natural abundance	50 (±0.3) mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.23 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KMC, Strand ID: A Detail

Release date

2009-11-30

Citation

The structure of the N-terminus of kindlin-1: a domain important for alphaiibbeta3 integrin activation
Goult, B.T., Bouaouina, M., Harburger, D.S., Bate, N., Patel, B., Anthis, N.J., Campbell, I.D., Calderwood, D.A., Barsukov, I.L., Roberts, G.C., Critchley, D.R.
J. Mol. Biol. (2009), 394, 944-956, PubMed 19804783 , DOI 10.1016/j.jmb.2009.09.061 , Abstract

Entries sharing articles Swiss-Prot: 2 entries Detail

  Swiss-Prot: P59113 released on 2002-12-06
    Title FERM1_MOUSE Entity Fermitin family homolog 1
  Swiss-Prot: Q9BQL6 released on 2002-12-06
    Title FERM1_HUMAN Entity Fermitin family homolog 1

Related entities 1. F0, : 1 : 2 : 35 entities Detail

Interaction partners 1. F0, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC