Barnase bound to d(CGAC) high pressure
VINTFDGVAD YLQTYHKLPD NYITKSEAQA LGWVASKGNL ADVAPGKSIG GDIFSNREGK LPGKSGRTWR EADINYTSGF RNSDRILYSS DWLIYKTTDA YQTFTKIR
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 41.5 % (269 of 648) | 41.5 % (269 of 648) |
Backbone | 64.6 % (144 of 223) | 64.6 % (144 of 223) |
Sidechain | 29.4 % (125 of 425) | 29.4 % (125 of 425) |
Aromatic | 0.0 % (0 of 68) | 0.0 % (0 of 68) |
Methyl | 90.9 % (50 of 55) | 90.9 % (50 of 55) |
1. barnase
VINTFDGVAD YLQTYHKLPD NYITKSEAQA LGWVASKGNL ADVAPGKSIG GDIFSNREGK LPGKSGRTWR EADINYTSGF RNSDRILYSS DWLIYKTTDA YQTFTKIRSolvent system 90% H2O/10% D2O, Pressure 2000 atm, Temperature 298 K, pH 6.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | barnase | [U-100% 13C; U-100% 15N] | 1.1 mM | |
2 | sodium phosphate | natural abundance | 30 mM | |
3 | d(CGAC) | natural abundance | 4.7 mM | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |