BMRBj - BMREntryMaster

Found 1 Document (1.155 seconds)

BMRB: 16237

2KGS

Solution structure of the amino-terminal domain of OmpATb, a pore forming protein from Mycobacterium tuberculosis.

Authors

Yang, Y., Auguin, D., Delbecq, S., Dumas, E., Molle, V., Saint, N.

Assembly

OmpATb

Entity

1. OmpATb (polymer, Thiol state: all disulfide bound), 254 monomers, 26067.12 Da Detail

GASALSLSLL SISRSGNTVT LIGDFPDEAA KAALMTALNG LLAPGVNVID QIHVDPVVRS LDFSSAEPVF TASVPIPDFG LKVERDTVTL TGTAPSSEHK DAVKRAATST WPDMKIVNNI EVTGQAPPGP PASGPCADLQ SAINAVTGGP IAFGNDGASL IPADYEILNR VADKLKACPD ARVTINGYTD NTGSEGINIP LSAQRAKIVA DYLVARGVAG DHIATVGLGS VNPIASNATP EGRAKNRRVE IVVN

Formula weight

26067.12 Da

Source organism

Mycobacterium tuberculosis

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing, minimization

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 79.0 %, Completeness (bb): 91.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	79.0 % (2135 of 2703)	87.0 % (1201 of 1380)	65.4 % (699 of 1068)	92.2 % (235 of 255)
Backbone	91.5 % (1360 of 1486)	97.5 % (498 of 511)	84.7 % (627 of 740)	100.0 % (235 of 235)
Sidechain	67.4 % (976 of 1449)	80.9 % (703 of 869)	48.8 % (273 of 560)	0.0 % (0 of 20)
Aromatic	14.3 % (14 of 98)	16.3 % (8 of 49)	12.5 % (6 of 48)	0.0 % (0 of 1)
Methyl	67.9 % (235 of 346)	94.8 % (164 of 173)	41.0 % (71 of 173)

1. amino-terminal domain

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 300 (±.1) K, pH 6 (±.2), Details 26.1kDa Cterminal fragment of OMPaTB (73-326)

#	Name	Isotope labeling	Type	Concentration
1	entity	natural abundance		0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance		50 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 300 (±.1) K, pH 6 (±.2), Details 26.1kDa Cterminal fragment of OMPaTB (73-326) 15N-enriched protein (U-99% 15N)

#	Name	Isotope labeling	Type	Concentration
3	entity	[U-99% 15N]		0.0 ~ 0.0 mM
4	sodium phosphate	natural abundance		50 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 300 (±.1) K, pH 6 (±.2), Details 26.1kDa Cterminal fragment of OMPaTB (73-326) 15N&13C-enriched protein (U-99% 15N U-99% 13C)

#	Name	Isotope labeling	Type	Concentration
5	entity	[U-99% 15N, U-99% 13C]		0.0 ~ 0.0 mM
6	sodium phosphate	natural abundance		50 mM

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 300 (±.1) K, pH 6 (±.2), Details 13.6kDa Nterminal fragment (73-204) of the Cterminal fragment of OMPaTB (73-326) 15N-enriched protein (U-99% 15N)

#	Name	Isotope labeling	Type	Concentration
7	entity	[U-99% 15N]		0.0 ~ 0.0 mM
8	sodium phosphate	natural abundance		50 mM

Protein Blocks Logo

Calculated from 10 models in PDB: 2KGS, Strand ID: A Detail

Release date

2011-05-31

Citation

Structure of the Mycobacterium tuberculosis OmpATb protein: a model of an oligomeric channel in the mycobacterial cell wall
Yang, Y., Auguin, D., Delbecq, S., Dumas, E., Molle, G., Molle, V., Roumestand, C., Saint, N.
Proteins (2011), 79, 645-661, PubMed 21117233 , DOI 10.1002/prot.22912 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P9WIU5 released on 2014-04-16
Title ARFA_MYCTU Entity Peptidoglycan-binding protein ArfA

Related entities 1. OmpATb, : 3 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-1H NOESY

Instrument

Bruker Avance - 600 MHz 5 mn Z-gradient 1H-13C-15N cryogenic probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 300 (±.1) K, pH 6 (±.2), Details 26.1kDa Cterminal fragment of OMPaTB (73-326)

#	Name	Isotope labeling	Type	Concentration
1	entity	natural abundance		0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance		50 mM

2 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz 5 mn Z-gradient 1H-13C-15N cryogenic probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 300 (±.1) K, pH 6 (±.2), Details 26.1kDa Cterminal fragment of OMPaTB (73-326) 15N-enriched protein (U-99% 15N)

#	Name	Isotope labeling	Type	Concentration
3	entity	[U-99% 15N]		0.0 ~ 0.0 mM
4	sodium phosphate	natural abundance		50 mM

3 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz 5 mn Z-gradient 1H-13C-15N cryogenic probe

Sample

#	Name	Isotope labeling	Type	Concentration
3	entity	[U-99% 15N]		0.0 ~ 0.0 mM
4	sodium phosphate	natural abundance		50 mM

4 3D HNCO

Instrument

Bruker Avance - 600 MHz 5 mn Z-gradient 1H-13C-15N cryogenic probe

Sample

#	Name	Isotope labeling	Type	Concentration
5	entity	[U-99% 15N, U-99% 13C]		0.0 ~ 0.0 mM
6	sodium phosphate	natural abundance		50 mM

5 3D HNCA

Instrument

Bruker Avance - 600 MHz 5 mn Z-gradient 1H-13C-15N cryogenic probe

Sample

#	Name	Isotope labeling	Type	Concentration
5	entity	[U-99% 15N, U-99% 13C]		0.0 ~ 0.0 mM
6	sodium phosphate	natural abundance		50 mM

6 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz 5 mn Z-gradient 1H-13C-15N cryogenic probe

Sample

#	Name	Isotope labeling	Type	Concentration
5	entity	[U-99% 15N, U-99% 13C]		0.0 ~ 0.0 mM
6	sodium phosphate	natural abundance		50 mM

7 3D 1H-13C NOESY

Instrument

Bruker Avance - 600 MHz 5 mn Z-gradient 1H-13C-15N cryogenic probe

Sample

#	Name	Isotope labeling	Type	Concentration
5	entity	[U-99% 15N, U-99% 13C]		0.0 ~ 0.0 mM
6	sodium phosphate	natural abundance		50 mM

8 2D 1H-15N HSQC

Instrument

Bruker Avance - 500 MHz 5 mn Z-gradient 1H-13C-15N cryogenic probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 300 (±.1) K, pH 6 (±.2), Details 13.6kDa Nterminal fragment (73-204) of the Cterminal fragment of OMPaTB (73-326) 15N-enriched protein (U-99% 15N)

#	Name	Isotope labeling	Type	Concentration
7	entity	[U-99% 15N]		0.0 ~ 0.0 mM
8	sodium phosphate	natural abundance		50 mM

9 2D 1H-1H NOESY

Instrument

Bruker Avance - 600 MHz 5 mn Z-gradient 1H-13C-15N cryogenic probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 300 (±0.1) K, pH 6 (±.2), Details 26.1kDa Cterminal fragment of OMPaTB (73-326)

#	Name	Isotope labeling	Type	Concentration
1	entity	natural abundance		0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance		50 mM

nullKeywords BON Domain, Mycobacterium tuberculosis, outer membrane protein A

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.155 seconds)

BMRB: 16237

Sample #1

Sample #2

Sample #3

Sample #4

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. OmpATb, : 3 entities Detail

Experiments performed 9 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample