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BMRB: 16266

2K3R

Solution Structure of RPP29-RPP21 complex from Pyrococcus furiosis

Authors

Xu, Y., Foster, M.P.

Assembly

Pfu RPP29-RPP21 complex

Entity

1. Pfu RPP29 (polymer, Thiol state: all free), 127 monomers, 14953.08 Da Detail

MWRNSEEREN RTSGRSQGSY QEIIGRTWIF RGAHRGRVNK KNIVWHELIG LKVRVVNSTH PGYVGIEGYV IDETRNMLVI AGENKVWKVP KDVCIFEFET WDGTKIKISG EKLVGRPEMR LKKRWRK

2. Pfu RPP21 (polymer, Thiol state: all other bound), 123 monomers, 14867.89 Da Detail

MAKYNEKKEK KRIAKERIDI LFSLAERVFP YSPELAKRYV ELALLVQQKA KVKIPRKWKR RYCKKCHAFL VPGINARVRL RQKRMPHIVV KCLECGHIMR YPYIKEIKKR RIEKMEYGGL VPR

3. ZN (non-polymer), 65.409 Da

Total weight

29886.377 Da

Max. entity weight

14953.08 Da

Source organism

Pyrococcus furiosus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.0 %, Completeness: 72.8 %, Completeness (bb): 86.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	72.8 % (2312 of 3178)	69.1 % (1172 of 1696)	76.4 % (933 of 1221)	79.3 % (207 of 261)
Backbone	86.3 % (1277 of 1480)	85.2 % (432 of 507)	87.2 % (639 of 733)	85.8 % (206 of 240)
Sidechain	64.3 % (1241 of 1931)	62.2 % (740 of 1189)	69.3 % (500 of 721)	4.8 % (1 of 21)
Aromatic	23.8 % (59 of 248)	41.1 % (51 of 124)	6.0 % (7 of 117)	14.3 % (1 of 7)
Methyl	93.1 % (242 of 260)	92.3 % (120 of 130)	93.8 % (122 of 130)

1. Pfu RPP29

MWRNSEEREN RTSGRSQGSY QEIIGRTWIF RGAHRGRVNK KNIVWHELIG LKVRVVNSTH PGYVGIEGYV IDETRNMLVI AGENKVWKVP KDVCIFEFET WDGTKIKISG EKLVGRPEMR LKKRWRK

2. Pfu RPP21

MAKYNEKKEK KRIAKERIDI LFSLAERVFP YSPELAKRYV ELALLVQQKA KVKIPRKWKR RYCKKCHAFL VPGINARVRL RQKRMPHIVV KCLECGHIMR YPYIKEIKKR RIEKMEYGGL VPR

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	Pfu RPP29	[U-100% 13C; U-100% 15N]	1.25 mM
2	Pfu RPP21	natural abundance	1.5 mM
3	TRIS	natural abundance	10 mM
4	potassium chloride	natural abundance	10 mM
5	Zinc chloride	natural abundance	0.3 mM
6	sodium azide	natural abundance	0.02 %
7	D2O	[U-99.8% 2H]	10 %
8	H2O	natural abundance	90 %
9	DSS	natural abundance	3 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 328 K, pH 5.8

#	Name	Isotope labeling	Concentration
10	Pfu RPP29	[U-100% 13C; U-100% 15N]	1.25 mM
11	Pfu RPP21	natural abundance	1.5 mM
12	TRIS	natural abundance	10 mM
13	potassium chloride	natural abundance	10 mM
14	Zinc chloride	natural abundance	0.3 %
15	sodium azide	natural abundance	0.02 %
16	D2O	[U-99.8% 2H]	100 %
17	DSS	natural abundance	3 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 5.8

#	Name	Isotope labeling	Concentration
18	Pfu RPP21	[U-100% 13C; U-100% 15N]	1 mM
19	Pfu RPP29	natural abundance	1.2 mM
20	TRIS	natural abundance	10 mM
21	potassium chloride	natural abundance	10 mM
22	Zinc chloride	natural abundance	0.3 mM
23	sodium azide	natural abundance	0.02 %
24	H2O	natural abundance	90 %
25	D2O	[U-99.8% 2H]	10 %
26	DSS	natural abundance	3 mM

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 328 K, pH 5.8

#	Name	Isotope labeling	Concentration
27	Pfu RPP21	[U-100% 13C; U-100% 15N]	1 mM
28	Pfu RPP29	natural abundance	1.2 mM
29	TRIS	natural abundance	10 mM
30	potassium chloride	natural abundance	10 mM
31	Zinc chloride	natural abundance	0.3 mM
32	sodium azide	natural abundance	0.02 %
33	D2O	[U-99.8% 2H]	100 %
34	DSS	natural abundance	3 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.101329

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.101329

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.101329

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.101329

Referencing offset: 0.16 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2KI7, Strand ID: A, B Detail

Release date

2009-09-10

Citation

Solution structure of an archaeal RNase P binary protein complex: formation of the 30-kDa complex between Pyrococcus furiosus RPP21 and RPP29 is accompanied by coupled protein folding and highlights critical features for protein-protein and protein-RNA interactions
Xu, Y., Amero, C.D., Pulukkunat, D.K., Gopalan, V., Foster, M.P.
J. Mol. Biol. (2009), 393, 1043-1055, PubMed 19733182 , DOI 10.1016/j.jmb.2009.08.068 , Abstract

Related entities 1. Pfu RPP29, : 1 : 10 entities Detail

Related entities 2. Pfu RPP21, : 1 : 2 : 19 entities Detail

Interaction partners 1. Pfu RPP29, : 1 : 1 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 20 experiments Detail

1 2D 1H-15N HSQC