BMRBj - BMREntryMaster

Found 1 Document (1.044 seconds)

BMRB: 16300

8JEX

intrinsically unfolded alpha-synuclein

Authors

Rao, J., Kim, Y.E., Park, L.S., Ulmer, T.S.

Assembly

alpha-synuclein

Entity

1. alpha-synuclein (polymer, Thiol state: not present), 140 monomers, 14459.97 Da Detail

MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA

Formula weight

14459.97 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.9 %, Completeness: 43.4 %, Completeness (bb): 75.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	43.4 % (650 of 1498)	19.6 % (151 of 771)	63.5 % (370 of 583)	89.6 % (129 of 144)
Backbone	75.5 % (627 of 830)	46.1 % (135 of 293)	90.3 % (363 of 402)	95.6 % (129 of 135)
Sidechain	16.3 % (129 of 790)	3.1 % (15 of 478)	37.6 % (114 of 303)	0.0 % (0 of 9)
Aromatic	0.0 % (0 of 56)	0.0 % (0 of 28)	0.0 % (0 of 28)
Methyl	15.8 % (25 of 158)	2.5 % (2 of 79)	29.1 % (23 of 79)

1. alpha-synuclein

MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 288.2 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	alpha-synuclein	[U-100% 13C; U-100% 15N; U-80% 2H]	0.5 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %
4	HEPES NaCl	natural abundance	25 mM
5	NaCl	natural abundance	50 mM
6	NaN3 solution	natural abundance	0.02 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.868 ppm	internal	indirect	0.2514495
¹H	water	protons	4.868 ppm	internal	direct	1.0
¹⁵N	water	protons	4.868 ppm	internal	indirect	0.101329

Referencing offset: 0.56 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.868 ppm	internal	indirect	0.2514495
¹H	water	protons	4.868 ppm	internal	direct	1.0
¹⁵N	water	protons	4.868 ppm	internal	indirect	0.101329

Referencing offset: 0.56 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.868 ppm	internal	indirect	0.2514495
¹H	water	protons	4.868 ppm	internal	direct	1.0
¹⁵N	water	protons	4.868 ppm	internal	indirect	0.101329

Referencing offset: 0.5 ppm, Outliers: 1 Detail

Release date

2009-06-02

Citation

Effect of pseudorepeat rearrangement on alpha-synuclein misfolding, vesicle binding, and micelle binding
Rao, J., Kim, Y.E., Park, L.S., Ulmer, T.S.
J. Mol. Biol. (2009), 390, 516-529, PubMed 19481090 , DOI 10.1016/j.jmb.2009.05.058 , Abstract

Related entities 1. alpha-synuclein, : 1 : 108 : 4 : 18 : 22 entities Detail

Interaction partners 1. alpha-synuclein, : 48 interactors Detail

More details for 48 interactors identified by 24 citations

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr16300_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr16300_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	OK	626		92.9 (chain: 1, length: 140)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 140, Sequence coverage: 92.9%
- Total number of assignments
  - ¹H chemical shifts: 130
  - ¹³C chemical shifts: 366
  - ¹⁵N chemical shifts: 130
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	771	130	16.9
¹³C chemical shifts	583	366	62.8
¹⁵N chemical shifts	144	130	90.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	293	130	44.4
¹³C chemical shifts	280	258	92.1
¹⁵N chemical shifts	135	130	96.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	478	0	0.0
¹³C chemical shifts	303	108	35.6
¹⁵N chemical shifts	9	0	0.0

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Found 1 Document (1.044 seconds)

BMRB: 16300

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. alpha-synuclein, : 1 : 108 : 4 : 18 : 22 entities Detail

Interaction partners 1. alpha-synuclein, : 48 interactors Detail

Experiments performed 4 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail