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SLAS-bound alpha-synuclein
Authors
Rao, J., Kim, Y.E., Park, L.S., Ulmer, T.S.
Assembly
monomer
Entity
1. monomer (polymer, Thiol state: not present), 140 monomers, 14459.97 Da Detail

MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA


Formula weight
14459.97 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 95.0 %, Completeness: 48.8 %, Completeness (bb): 79.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All48.8 % (731 of 1498)26.1 % (201 of 771)68.1 % (397 of 583)92.4 % (133 of 144)
Backbone79.6 % (661 of 830)51.2 % (150 of 293)94.0 % (378 of 402)98.5 % (133 of 135)
Sidechain23.3 % (184 of 790)10.7 % (51 of 478)43.9 % (133 of 303) 0.0 % (0 of 9)
Aromatic 5.4 % (3 of 56) 0.0 % (0 of 28)10.7 % (3 of 28)
Methyl25.3 % (40 of 158)16.5 % (13 of 79)34.2 % (27 of 79)

1. alpha-synuclein

MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298.2 K, pH 7.4


#NameIsotope labelingTypeConcentration
1alpha-synuclein[U-100% 13C; U-100% 15N; U-80% 2H]0.75 mM
2sodium lauroyl sarcosinatenatural abundance75 mM
3H2Onatural abundance95 %
4D2Onatural abundance5 %

LACS Plot; CA
Referencing offset: 0.75 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 0.75 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.44 ppm, Outliers: 1 Detail
Release date
2009-06-02
Citation
Effect of pseudorepeat rearrangement on alpha-synuclein misfolding, vesicle binding, and micelle binding
Rao, J., Kim, Y.E., Park, L.S., Ulmer, T.S.
J. Mol. Biol. (2009), 390, 516-529, PubMed 19481090 , DOI 10.1016/j.jmb.2009.05.058 ,
Entries sharing articles BMRB: 4 entries Detail
  BMRB: 16300 released on 2009-06-02
    Title intrinsically unfolded alpha-synuclein
  BMRB: 16301 released on 2009-06-02
    Title intrinsically unfolded alpha-synuclein variant SaS
  BMRB: 16303 released on 2009-06-02
    Title SLAS-bound SaS synuclein variant
  BMRB: 16304 released on 2009-06-02
    Title SLAS-bound beta-synuclein
Related entities 1. monomer, : 1 : 108 : 4 : 18 : 22 entities Detail
Interaction partners 1. monomer, : 48 interactors Detail
Experiments performed 4 experiments Detail
Chemical shift validation 3 contents Detail