BMRB: 16302

SLAS-bound alpha-synuclein

Authors

Rao, J., Kim, Y.E., Park, L.S., Ulmer, T.S.

Assembly

monomer

Entity

1. monomer (polymer, Thiol state: not present), 140 monomers, 14459.97 Da Detail

MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA

Formula weight

14459.97 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.0 %, Completeness: 48.8 %, Completeness (bb): 79.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	48.8 % (731 of 1498)	26.1 % (201 of 771)	68.1 % (397 of 583)	92.4 % (133 of 144)
Backbone	79.6 % (661 of 830)	51.2 % (150 of 293)	94.0 % (378 of 402)	98.5 % (133 of 135)
Sidechain	23.3 % (184 of 790)	10.7 % (51 of 478)	43.9 % (133 of 303)	0.0 % (0 of 9)
Aromatic	5.4 % (3 of 56)	0.0 % (0 of 28)	10.7 % (3 of 28)
Methyl	25.3 % (40 of 158)	16.5 % (13 of 79)	34.2 % (27 of 79)

1. alpha-synuclein

MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298.2 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	alpha-synuclein	[U-100% 13C; U-100% 15N; U-80% 2H]		0.75 mM
2	sodium lauroyl sarcosinate	natural abundance		75 mM
3	H2O	natural abundance		95 %
4	D2O	natural abundance		5 %

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LACS Plot; CA

Referencing offset: 0.75 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.771 ppm	internal	indirect	0.2514495
1H	water	protons	4.771 ppm	internal	direct	1.0
15N	water	protons	4.771 ppm	internal	indirect	0.101329

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LACS Plot; CB

Referencing offset: 0.75 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.771 ppm	internal	indirect	0.2514495
1H	water	protons	4.771 ppm	internal	direct	1.0
15N	water	protons	4.771 ppm	internal	indirect	0.101329

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LACS Plot; CO

Referencing offset: 0.44 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.771 ppm	internal	indirect	0.2514495
1H	water	protons	4.771 ppm	internal	direct	1.0
15N	water	protons	4.771 ppm	internal	indirect	0.101329

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Release date

2009-06-02

Citation

Effect of pseudorepeat rearrangement on alpha-synuclein misfolding, vesicle binding, and micelle binding
Rao, J., Kim, Y.E., Park, L.S., Ulmer, T.S.
J. Mol. Biol. (2009), 390, 516-529, PubMed 19481090 , DOI 10.1016/j.jmb.2009.05.058 , Abstract

Entries sharing articles BMRB: 4 entries Detail

Related entities 1. monomer, : 1 : 108 : 4 : 18 : 22 entities Detail

Interaction partners 1. monomer, : 48 interactors Detail

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail