BMRBj - BMREntryMaster

Found 1 Document (0.419 seconds)

BMRB: 16304

SLAS-bound beta-synuclein

Authors

Rao, J., Kim, Y.E., Park, L.S., Ulmer, T.S.

Assembly

monomer

Entity

1. monomer (polymer, Thiol state: not present), 134 monomers, 14287.70 Da Detail

MDVFMKGLSM AKEGVVAAAE KTKQGVTEAA EKTKEGVLYV GSKTREGVVQ GVASVAEKTK EQASHLGGAV FSGAGNIAAA TGLVKREEFP TDLKPEEVAQ EAAEEPLIEP LMEPEGESYE DPPQEEYQEY EPEA

Formula weight

14287.7 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.5 %, Completeness: 45.6 %, Completeness (bb): 78.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	45.6 % (672 of 1473)	24.3 % (186 of 766)	63.2 % (363 of 574)	92.5 % (123 of 133)
Backbone	78.0 % (615 of 788)	51.6 % (141 of 273)	90.2 % (351 of 389)	97.6 % (123 of 126)
Sidechain	20.5 % (165 of 806)	9.1 % (45 of 493)	39.2 % (120 of 306)	0.0 % (0 of 7)
Aromatic	3.0 % (2 of 66)	3.0 % (1 of 33)	3.0 % (1 of 33)
Methyl	19.6 % (27 of 138)	7.2 % (5 of 69)	31.9 % (22 of 69)

1. beta-synuclein

MDVFMKGLSM AKEGVVAAAE KTKQGVTEAA EKTKEGVLYV GSKTREGVVQ GVASVAEKTK EQASHLGGAV FSGAGNIAAA TGLVKREEFP TDLKPEEVAQ EAAEEPLIEP LMEPEGESYE DPPQEEYQEY EPEA

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298.2 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	beta-synuclein	[U-100% 13C; U-100% 15N; U-80% 2H]	0.75 mM
2	sodium lauroyl sarcosinate	natural abundance	75 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.771 ppm	internal	indirect	0.2514495
¹H	water	protons	4.771 ppm	internal	direct	1.0
¹⁵N	water	protons	4.771 ppm	internal	indirect	0.101329

Referencing offset: 0.7 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.771 ppm	internal	indirect	0.2514495
¹H	water	protons	4.771 ppm	internal	direct	1.0
¹⁵N	water	protons	4.771 ppm	internal	indirect	0.101329

Referencing offset: 0.7 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.771 ppm	internal	indirect	0.2514495
¹H	water	protons	4.771 ppm	internal	direct	1.0
¹⁵N	water	protons	4.771 ppm	internal	indirect	0.101329

Referencing offset: 0.57 ppm, Outliers: 4 Detail

Release date

2009-06-02

Citation

Effect of pseudorepeat rearrangement on alpha-synuclein misfolding, vesicle binding, and micelle binding
Rao, J., Kim, Y.E., Park, L.S., Ulmer, T.S.
J. Mol. Biol. (2009), 390, 516-529, PubMed 19481090 , DOI 10.1016/j.jmb.2009.05.058 , Abstract

Related entities 1. monomer, : 2 : 150 entities Detail

Interaction partners 1. monomer, : 3 interactors Detail

More details for 3 interactors identified by 3 citations

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr16304_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr16304_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	OK	588		91.0 (chain: 1, length: 134)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 134, Sequence coverage: 91.0%
- Total number of assignments
  - ¹H chemical shifts: 122
  - ¹³C chemical shifts: 344
  - ¹⁵N chemical shifts: 122
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	766	122	15.9
¹³C chemical shifts	574	344	59.9
¹⁵N chemical shifts	135	122	90.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	273	122	44.7
¹³C chemical shifts	268	239	89.2
¹⁵N chemical shifts	126	122	96.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	493	0	0.0
¹³C chemical shifts	306	105	34.3
¹⁵N chemical shifts	9	0	0.0

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Found 1 Document (0.419 seconds)

BMRB: 16304

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. monomer, : 2 : 150 entities Detail

Interaction partners 1. monomer, : 3 interactors Detail

Experiments performed 4 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail