1H, 15N, and 13C resonance assignments of the 2/2 hemoglobin from the cyanobacterium Synechococcus sp. PCC 7002 in the ferric bis-histidine state
ASLYEKLGGA AAVDLAVEKF YGKVLADERV NRFFVNTDMA KQKQHQKDFM TYAFGGTDRF PGRSMRAAHQ DLVENAGLTD VHFDAIAENL VLTLQELNVS QDLIDEVVTI VGSVQHRNDV LNR
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | covalent | sing | 1:HIS116:NE2 | 2:HEB1:CAB |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 89.8 % (1265 of 1409) | 87.7 % (635 of 724) | 90.3 % (496 of 549) | 98.5 % (134 of 136) |
Backbone | 94.8 % (698 of 736) | 93.3 % (236 of 253) | 94.7 % (342 of 361) | 98.4 % (120 of 122) |
Sidechain | 85.9 % (677 of 788) | 84.7 % (399 of 471) | 87.1 % (264 of 303) | 100.0 % (14 of 14) |
Aromatic | 90.9 % (100 of 110) | 92.7 % (51 of 55) | 89.1 % (49 of 55) | |
Methyl | 99.4 % (153 of 154) | 98.7 % (76 of 77) | 100.0 % (77 of 77) |
1. GlbN
ASLYEKLGGA AAVDLAVEKF YGKVLADERV NRFFVNTDMA KQKQHQKDFM TYAFGGTDRF PGRSMRAAHQ DLVENAGLTD VHFDAIAENL VLTLQELNVS QDLIDEVVTI VGSVQHRNDV LNRSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | GlbN | natural abundance | 1.0 ~ 2.0 mM | |
2 | H2O | natural abundance | 90 % | |
3 | D2O | natural abundance | 10 % | |
4 | phosphate buffer | natural abundance | 20 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | GlbN | [U-15N] | 1.0 ~ 2.0 mM | |
6 | H2O | natural abundance | 90 % | |
7 | D2O | natural abundance | 10 % | |
8 | phosphate buffer | natural abundance | 20 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | GlbN | [U-13C; U-15N] | 0.6 ~ 1.4 mM | |
10 | H2O | natural abundance | 90 % | |
11 | D2O | natural abundance | 10 % | |
12 | phosphate buffer | natural abundance | 20 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | GlbN | natural abundance | 1.0 ~ 4.0 mM | |
14 | phosphate buffer | natural abundance | 20 mM | |
15 | D2O | natural abundance | 100 % |