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BMRB: 16311

2KJ4

Solution structure of the complex of VEK-30 and plasminogen kringle 2

Authors

Wang, M., Zajicek, J., Prorok, M., Castellin, F.J.

Assembly

VEK-30/plasminogen kringle 2 complex

Entity

1. plasminogen kringle 2 (polymer, Thiol state: all disulfide bound), 87 monomers, 10151.22 Da Detail

YVEFSEECMH GSGENYDGKI SKTMSGLECQ AWDSQSPHAH GYIPSKFPNK NLKKNYCRNP DRDLRPWCFT TDPNKRWEYC DIPRCAA

2. VEK-30 (polymer, Thiol state: not present), 32 monomers, 3772.093 Da Detail

GSVEKLTADA ELQRLKNERH EEAELERLKS EY

Total weight

13923.3125 Da

Max. entity weight

10151.22 Da

Entity Connection

disulfide 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS8:SG	1:CYS85:SG
2	disulfide	sing	1:CYS29:SG	1:CYS68:SG
3	disulfide	sing	1:CYS57:SG	1:CYS80:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.3 %, Completeness: 96.9 %, Completeness (bb): 96.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.9 % (1360 of 1404)	96.6 % (719 of 744)	97.4 % (521 of 535)	96.0 % (120 of 125)
Backbone	96.9 % (678 of 700)	95.4 % (226 of 237)	98.0 % (344 of 351)	96.4 % (108 of 112)
Sidechain	97.2 % (794 of 817)	97.2 % (493 of 507)	97.3 % (289 of 297)	92.3 % (12 of 13)
Aromatic	93.1 % (121 of 130)	93.8 % (61 of 65)	91.9 % (57 of 62)	100.0 % (3 of 3)
Methyl	100.0 % (74 of 74)	100.0 % (37 of 37)	100.0 % (37 of 37)

1. plasminogen kringle 2

YVEFSEECMH GSGENYDGKI SKTMSGLECQ AWDSQSPHAH GYIPSKFPNK NLKKNYCRNP DRDLRPWCFT TDPNKRWEYC DIPRCAA

2. VEK-30

GSVEKLTADA ELQRLKNERH EEAELERLKS EY

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.2) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	entity_1	[U-13C; U-15N]	1 (±0.1) mM
2	entity_2	natural abundance	1 (±0.1) mM
3	HEPES	[U-100% 2H]	50 mM
4	sodium chloride	natural abundance	200 mM
5	sodium azide	natural abundance	3 mM
6	DSS	natural abundance	0.2 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.2) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
9	entity_1	natural abundance	1 mM
10	entity_2	[U-15N]	1 mM
11	HEPES	[U-100% 2H]	50 mM
12	sodium chloride	natural abundance	200 mM
13	sodium azide	natural abundance	3 mM
14	DSS	natural abundance	0.2 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2KJ4, Strand ID: A, B Detail

Release date

2009-10-13

Citation

Solution structure of the complex of VEK-30 and plasminogen kringle 2
Wang, M., Zajicek, J., Geiger, J.H., Prorok, M., Castellino, F.J.
J. Struct. Biol. (2010), 169, 349-359, PubMed 19800007 , DOI 10.1016/j.jsb.2009.09.011 , Abstract

Related entities 1. plasminogen kringle 2, : 116 entities Detail

Related entities 2. VEK-30, : 1 : 12 : 3 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC