Solution NMR structure of a domain from a putative phage integrase protein Nmul_A0064 from Nitrosospira multiformis, Northeast Structural Genomics Consortium Target NmR46C.
MAEKNAYTVA QLADEYFERM IAGRWKHPNI VRSRIEKDIK PAIGSLKVED VKPRHIDDVL KAVMKRGAPS IANDTLRWLK RMFNYAIKRH IIEYNPAAAF DPGDAGGKLE HHHHHH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 89.2 % (1238 of 1388) | 96.7 % (704 of 728) | 76.9 % (417 of 542) | 99.2 % (117 of 118) |
Backbone | 85.7 % (586 of 684) | 99.1 % (230 of 232) | 72.2 % (247 of 342) | 99.1 % (109 of 110) |
Sidechain | 93.5 % (761 of 814) | 95.6 % (474 of 496) | 90.0 % (279 of 310) | 100.0 % (8 of 8) |
Aromatic | 63.1 % (77 of 122) | 73.8 % (45 of 61) | 50.8 % (30 of 59) | 100.0 % (2 of 2) |
Methyl | 100.0 % (120 of 120) | 100.0 % (60 of 60) | 100.0 % (60 of 60) |
1. NmR46C
MAEKNAYTVA QLADEYFERM IAGRWKHPNI VRSRIEKDIK PAIGSLKVED VKPRHIDDVL KAVMKRGAPS IANDTLRWLK RMFNYAIKRH IIEYNPAAAF DPGDAGGKLE HHHHHHSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | NmR46C | [U-98% 13C; U-98% 15N] | 1.2 mM | |
2 | D2O | natural abundance | 10 % | |
3 | H2O | natural abundance | 90 % | |
4 | MES | natural abundance | 20 mM | |
5 | sodium chloride | natural abundance | 200 mM | |
6 | calcium chloride | natural abundance | 5 mM | |
7 | DTT | natural abundance | 10 mM | |
8 | DSS | natural abundance | 50 uM | |
9 | protease inhibitors | natural abundance | 1x v/v | |
10 | sodium azide | natural abundance | 0.02 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | NmR46C | [U-5% 13C; U-98% 15N] | 1.2 mM | |
12 | D2O | natural abundance | 10 % | |
13 | H2O | natural abundance | 90 % | |
14 | MES | natural abundance | 20 mM | |
15 | sodium chloride | natural abundance | 200 mM | |
16 | calcium chloride | natural abundance | 5 mM | |
17 | DTT | natural abundance | 10 mM | |
18 | DSS | natural abundance | 50 uM | |
19 | protease inhibitors | natural abundance | 1x v/v | |
20 | sodium azide | natural abundance | 0.02 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details aligned sample for RDC measurements; uses PEG/hexanol
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
21 | NmR46C | [U-5% 13C; U-98% 15N] | 1.2 mM | |
22 | D2O | natural abundance | 10 % | |
23 | H2O | natural abundance | 90 % | |
24 | MES | natural abundance | 20 mM | |
25 | sodium chloride | natural abundance | 200 mM | |
26 | calcium chloride | natural abundance | 5 mM | |
27 | DTT | natural abundance | 10 mM | |
28 | DSS | natural abundance | 50 uM | |
29 | protease inhibitors | natural abundance | 1x v/v | |
30 | sodium azide | natural abundance | 0.02 % |