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BMRB: 16313

2KJ6

NMR Solution Structure of a Tubulin folding cofactor B obtained from Arabidopsis thaliana: Northeast Structural Genomics Consortium target AR3436A

Authors

Mani, R., Gurla, S.V.T., Shastry, R., Foote, E., Ciccosanti, C., Jiang, M., Xiao, R., Nair, R., Everett, J., Huang, Y., Acton, T., Rost, B., Montelione, G.

Assembly

AR3436A

Entity

1. AR3436A (polymer, Thiol state: all free), 97 monomers, 10831.97 Da Detail

MGHHHHHHSH GDDSVHLHIT HANLKSFSAD ARFSPQMSVE AVKEKLWKKC GTSVNSMALE LYDDSGSKVA VLSDDSRPLG FFSPFDGFRL HIIDLDP

Formula weight

10831.97 Da

Source organism

Arabidopsis thaliana

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.7 %, Completeness: 84.4 %, Completeness (bb): 88.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.4 % (929 of 1101)	83.6 % (473 of 566)	84.2 % (369 of 438)	89.7 % (87 of 97)
Backbone	88.2 % (506 of 574)	88.3 % (173 of 196)	87.7 % (250 of 285)	89.2 % (83 of 93)
Sidechain	80.4 % (497 of 618)	78.9 % (292 of 370)	82.4 % (201 of 244)	100.0 % (4 of 4)
Aromatic	62.9 % (78 of 124)	62.9 % (39 of 62)	62.3 % (38 of 61)	100.0 % (1 of 1)
Methyl	98.9 % (87 of 88)	97.7 % (43 of 44)	100.0 % (44 of 44)

1. AR3436A

MGHHHHHHSH GDDSVHLHIT HANLKSFSAD ARFSPQMSVE AVKEKLWKKC GTSVNSMALE LYDDSGSKVA VLSDDSRPLG FFSPFDGFRL HIIDLDP

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.78mM, 5%D2O, 0.02%NaN3, 100mM DTT, 5mM CaCl2, 10mM NaCl, 20mM MES, pH 6.5

#	Name	Isotope labeling	Concentration
1	AR3436A	[U-100% 13C; U-100% 15N]	0.78 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %
4	NaN3	natural abundance	0.02 %
5	DTT	natural abundance	100 mM
6	CaCl2	natural abundance	5 mM
7	NaCl	natural abundance	10 mM
8	MES	natural abundance	20 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.95mM, 5%D2O, 0.02%NaN3, 100mM DTT, 5mM CaCl2, 10mM NaCl, 20mM MES, pH 6.5

#	Name	Isotope labeling	Concentration
9	AR3436A	[U-100% 13C; U-100% 15N]	0.95 mM
10	H2O	natural abundance	95 %
11	D2O	natural abundance	5 %
12	NaN3	natural abundance	0.02 %
13	DTT	natural abundance	100 mM
14	CaCl2	natural abundance	5 mM
15	NaCl	natural abundance	10 mM
16	MES	natural abundance	20 mM

Sample #3

Solvent system 95% H2O/15% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.4mM, 5%D2O, 0.02%NaN3, 100mM DTT, 5mM CaCl2, 10mM NaCl, 20mM MES, pH 6.5

#	Name	Isotope labeling	Concentration
17	AR3436A	[U-100% 13C; U-100% 15N]	0.4 mM
18	H2O	natural abundance	95 %
19	D2O	natural abundance	5 %
20	NaN3	natural abundance	0.02 %
21	DTT	natural abundance	100 mM
22	CaCl2	natural abundance	5 mM
23	NaCl	natural abundance	10 mM
24	MES	natural abundance	20 mM

Sample #4

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.4mM, 5%D2O, 0.02%NaN3, 100mM DTT, 5mM CaCl2, 10mM NaCl, 20mM MES, pH 6.5

#	Name	Isotope labeling	Concentration
25	AR3436A	[U-10% 13C; U-100% 15N]	0.56 mM
26	H2O	natural abundance	95 %
27	D2O	natural abundance	5 %
28	NaN3	natural abundance	0.02 %
29	DTT	natural abundance	100 mM
30	CaCl2	natural abundance	5 mM
31	NaCl	natural abundance	10 mM
32	MES	natural abundance	20 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.3 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KJ6, Strand ID: A Detail

Release date

2009-10-19

Citation

NMR Solution Structure of Tbulin folding Cofactor B obtained from Arabidopsis thaliana: Northeast Structural Genomics Consortium
Mani, R., Gurla, S.V.T., Shastry, R., Foote, E., Ciccosanti, C., Jiang, M., Xiao, R., Nair, R., Everett, J., Huang, Y., Acton, T., Rost, B., Montelione, G.
Not known

Related entities 1. AR3436A, : 1 : 2 : 17 entities Detail

Experiments performed 20 experiments Detail

1 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/15% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.4mM, 5%D2O, 0.02%NaN3, 100mM DTT, 5mM CaCl2, 10mM NaCl, 20mM MES, pH 6.5

#	Name	Isotope labeling	Concentration
17	AR3436A	[U-100% 13C; U-100% 15N]	0.4 mM
18	H2O	natural abundance	95 %
19	D2O	natural abundance	5 %
20	NaN3	natural abundance	0.02 %
21	DTT	natural abundance	100 mM
22	CaCl2	natural abundance	5 mM
23	NaCl	natural abundance	10 mM
24	MES	natural abundance	20 mM

2 3D 1H-13Caliphatic NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.78mM, 5%D2O, 0.02%NaN3, 100mM DTT, 5mM CaCl2, 10mM NaCl, 20mM MES, pH 6.5

#	Name	Isotope labeling	Concentration
1	AR3436A	[U-100% 13C; U-100% 15N]	0.78 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %
4	NaN3	natural abundance	0.02 %
5	DTT	natural abundance	100 mM
6	CaCl2	natural abundance	5 mM
7	NaCl	natural abundance	10 mM
8	MES	natural abundance	20 mM

3 3D 1H-13C aromatic NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.78mM, 5%D2O, 0.02%NaN3, 100mM DTT, 5mM CaCl2, 10mM NaCl, 20mM MES, pH 6.5

#	Name	Isotope labeling	Concentration
1	AR3436A	[U-100% 13C; U-100% 15N]	0.78 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %
4	NaN3	natural abundance	0.02 %
5	DTT	natural abundance	100 mM
6	CaCl2	natural abundance	5 mM
7	NaCl	natural abundance	10 mM
8	MES	natural abundance	20 mM

4 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.78mM, 5%D2O, 0.02%NaN3, 100mM DTT, 5mM CaCl2, 10mM NaCl, 20mM MES, pH 6.5

#	Name	Isotope labeling	Concentration
1	AR3436A	[U-100% 13C; U-100% 15N]	0.78 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %
4	NaN3	natural abundance	0.02 %
5	DTT	natural abundance	100 mM
6	CaCl2	natural abundance	5 mM
7	NaCl	natural abundance	10 mM
8	MES	natural abundance	20 mM

5 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.78mM, 5%D2O, 0.02%NaN3, 100mM DTT, 5mM CaCl2, 10mM NaCl, 20mM MES, pH 6.5

#	Name	Isotope labeling	Concentration
1	AR3436A	[U-100% 13C; U-100% 15N]	0.78 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %
4	NaN3	natural abundance	0.02 %
5	DTT	natural abundance	100 mM
6	CaCl2	natural abundance	5 mM
7	NaCl	natural abundance	10 mM
8	MES	natural abundance	20 mM

6 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/15% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.4mM, 5%D2O, 0.02%NaN3, 100mM DTT, 5mM CaCl2, 10mM NaCl, 20mM MES, pH 6.5

#	Name	Isotope labeling	Concentration
17	AR3436A	[U-100% 13C; U-100% 15N]	0.4 mM
18	H2O	natural abundance	95 %
19	D2O	natural abundance	5 %
20	NaN3	natural abundance	0.02 %
21	DTT	natural abundance	100 mM
22	CaCl2	natural abundance	5 mM
23	NaCl	natural abundance	10 mM
24	MES	natural abundance	20 mM

7 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/15% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.4mM, 5%D2O, 0.02%NaN3, 100mM DTT, 5mM CaCl2, 10mM NaCl, 20mM MES, pH 6.5

#	Name	Isotope labeling	Concentration
17	AR3436A	[U-100% 13C; U-100% 15N]	0.4 mM
18	H2O	natural abundance	95 %
19	D2O	natural abundance	5 %
20	NaN3	natural abundance	0.02 %
21	DTT	natural abundance	100 mM
22	CaCl2	natural abundance	5 mM
23	NaCl	natural abundance	10 mM
24	MES	natural abundance	20 mM

8 3D HBHA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/15% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.4mM, 5%D2O, 0.02%NaN3, 100mM DTT, 5mM CaCl2, 10mM NaCl, 20mM MES, pH 6.5

#	Name	Isotope labeling	Concentration
17	AR3436A	[U-100% 13C; U-100% 15N]	0.4 mM
18	H2O	natural abundance	95 %
19	D2O	natural abundance	5 %
20	NaN3	natural abundance	0.02 %
21	DTT	natural abundance	100 mM
22	CaCl2	natural abundance	5 mM
23	NaCl	natural abundance	10 mM
24	MES	natural abundance	20 mM

9 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/15% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.4mM, 5%D2O, 0.02%NaN3, 100mM DTT, 5mM CaCl2, 10mM NaCl, 20mM MES, pH 6.5

#	Name	Isotope labeling	Concentration
17	AR3436A	[U-100% 13C; U-100% 15N]	0.4 mM
18	H2O	natural abundance	95 %
19	D2O	natural abundance	5 %
20	NaN3	natural abundance	0.02 %
21	DTT	natural abundance	100 mM
22	CaCl2	natural abundance	5 mM
23	NaCl	natural abundance	10 mM
24	MES	natural abundance	20 mM

10 (4,3)D GFT-HNNCABCA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/15% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.4mM, 5%D2O, 0.02%NaN3, 100mM DTT, 5mM CaCl2, 10mM NaCl, 20mM MES, pH 6.5

#	Name	Isotope labeling	Concentration
17	AR3436A	[U-100% 13C; U-100% 15N]	0.4 mM
18	H2O	natural abundance	95 %
19	D2O	natural abundance	5 %
20	NaN3	natural abundance	0.02 %
21	DTT	natural abundance	100 mM
22	CaCl2	natural abundance	5 mM
23	NaCl	natural abundance	10 mM
24	MES	natural abundance	20 mM

11 (4,3)D GFT-CABCACA(CO)NHN

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/15% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.4mM, 5%D2O, 0.02%NaN3, 100mM DTT, 5mM CaCl2, 10mM NaCl, 20mM MES, pH 6.5

#	Name	Isotope labeling	Concentration
17	AR3436A	[U-100% 13C; U-100% 15N]	0.4 mM
18	H2O	natural abundance	95 %
19	D2O	natural abundance	5 %
20	NaN3	natural abundance	0.02 %
21	DTT	natural abundance	100 mM
22	CaCl2	natural abundance	5 mM
23	NaCl	natural abundance	10 mM
24	MES	natural abundance	20 mM

12 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/15% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.4mM, 5%D2O, 0.02%NaN3, 100mM DTT, 5mM CaCl2, 10mM NaCl, 20mM MES, pH 6.5

#	Name	Isotope labeling	Concentration
17	AR3436A	[U-100% 13C; U-100% 15N]	0.4 mM
18	H2O	natural abundance	95 %
19	D2O	natural abundance	5 %
20	NaN3	natural abundance	0.02 %
21	DTT	natural abundance	100 mM
22	CaCl2	natural abundance	5 mM
23	NaCl	natural abundance	10 mM
24	MES	natural abundance	20 mM

13 3D HNCA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/15% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.4mM, 5%D2O, 0.02%NaN3, 100mM DTT, 5mM CaCl2, 10mM NaCl, 20mM MES, pH 6.5

#	Name	Isotope labeling	Concentration
17	AR3436A	[U-100% 13C; U-100% 15N]	0.4 mM
18	H2O	natural abundance	95 %
19	D2O	natural abundance	5 %
20	NaN3	natural abundance	0.02 %
21	DTT	natural abundance	100 mM
22	CaCl2	natural abundance	5 mM
23	NaCl	natural abundance	10 mM
24	MES	natural abundance	20 mM

14 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.95mM, 5%D2O, 0.02%NaN3, 100mM DTT, 5mM CaCl2, 10mM NaCl, 20mM MES, pH 6.5

#	Name	Isotope labeling	Concentration
9	AR3436A	[U-100% 13C; U-100% 15N]	0.95 mM
10	H2O	natural abundance	95 %
11	D2O	natural abundance	5 %
12	NaN3	natural abundance	0.02 %
13	DTT	natural abundance	100 mM
14	CaCl2	natural abundance	5 mM
15	NaCl	natural abundance	10 mM
16	MES	natural abundance	20 mM

15 3D HNHA

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.4mM, 5%D2O, 0.02%NaN3, 100mM DTT, 5mM CaCl2, 10mM NaCl, 20mM MES, pH 6.5

#	Name	Isotope labeling	Concentration
25	AR3436A	[U-10% 13C; U-100% 15N]	0.56 mM
26	H2O	natural abundance	95 %
27	D2O	natural abundance	5 %
28	NaN3	natural abundance	0.02 %
29	DTT	natural abundance	100 mM
30	CaCl2	natural abundance	5 mM
31	NaCl	natural abundance	10 mM
32	MES	natural abundance	20 mM

16 3D H(CCO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/15% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.4mM, 5%D2O, 0.02%NaN3, 100mM DTT, 5mM CaCl2, 10mM NaCl, 20mM MES, pH 6.5

#	Name	Isotope labeling	Concentration
17	AR3436A	[U-100% 13C; U-100% 15N]	0.4 mM
18	H2O	natural abundance	95 %
19	D2O	natural abundance	5 %
20	NaN3	natural abundance	0.02 %
21	DTT	natural abundance	100 mM
22	CaCl2	natural abundance	5 mM
23	NaCl	natural abundance	10 mM
24	MES	natural abundance	20 mM

17 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/15% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.4mM, 5%D2O, 0.02%NaN3, 100mM DTT, 5mM CaCl2, 10mM NaCl, 20mM MES, pH 6.5

#	Name	Isotope labeling	Concentration
17	AR3436A	[U-100% 13C; U-100% 15N]	0.4 mM
18	H2O	natural abundance	95 %
19	D2O	natural abundance	5 %
20	NaN3	natural abundance	0.02 %
21	DTT	natural abundance	100 mM
22	CaCl2	natural abundance	5 mM
23	NaCl	natural abundance	10 mM
24	MES	natural abundance	20 mM

18 (4,3)D GFT-HABCAB(CO)NHN

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/15% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.4mM, 5%D2O, 0.02%NaN3, 100mM DTT, 5mM CaCl2, 10mM NaCl, 20mM MES, pH 6.5

#	Name	Isotope labeling	Concentration
17	AR3436A	[U-100% 13C; U-100% 15N]	0.4 mM
18	H2O	natural abundance	95 %
19	D2O	natural abundance	5 %
20	NaN3	natural abundance	0.02 %
21	DTT	natural abundance	100 mM
22	CaCl2	natural abundance	5 mM
23	NaCl	natural abundance	10 mM
24	MES	natural abundance	20 mM

19 3D C(CO)NH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/15% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.4mM, 5%D2O, 0.02%NaN3, 100mM DTT, 5mM CaCl2, 10mM NaCl, 20mM MES, pH 6.5

#	Name	Isotope labeling	Concentration
17	AR3436A	[U-100% 13C; U-100% 15N]	0.4 mM
18	H2O	natural abundance	95 %
19	D2O	natural abundance	5 %
20	NaN3	natural abundance	0.02 %
21	DTT	natural abundance	100 mM
22	CaCl2	natural abundance	5 mM
23	NaCl	natural abundance	10 mM
24	MES	natural abundance	20 mM

20 3D CCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/15% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.4mM, 5%D2O, 0.02%NaN3, 100mM DTT, 5mM CaCl2, 10mM NaCl, 20mM MES, pH 6.5

#	Name	Isotope labeling	Concentration
17	AR3436A	[U-100% 13C; U-100% 15N]	0.4 mM
18	H2O	natural abundance	95 %
19	D2O	natural abundance	5 %
20	NaN3	natural abundance	0.02 %
21	DTT	natural abundance	100 mM
22	CaCl2	natural abundance	5 mM
23	NaCl	natural abundance	10 mM
24	MES	natural abundance	20 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr16313_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr16313_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1003		88.7 (chain: 1, length: 97)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 97, Sequence coverage: 88.7%
- Total number of assignments
  - ¹H chemical shifts: 551
  - ¹³C chemical shifts: 366
  - ¹⁵N chemical shifts: 86
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	566	465	82.2
¹³C chemical shifts	438	366	83.6
¹⁵N chemical shifts	100	86	86.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	196	173	88.3
¹³C chemical shifts	194	167	86.1
¹⁵N chemical shifts	93	82	88.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	370	292	78.9
¹³C chemical shifts	244	199	81.6
¹⁵N chemical shifts	7	4	57.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	47	43	91.5
¹³C chemical shifts	47	44	93.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	62	36	58.1
¹³C chemical shifts	61	35	57.4
¹⁵N chemical shifts	1	1	100.0

Keywords AR3436A, Arabidopsis thaliana, NESG, Solution NMR, Tubulin folding cofactor B

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Found 1 Document (0.654 seconds)

BMRB: 16313

Sample #1

Sample #2

Sample #3

Sample #4

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. AR3436A, : 1 : 2 : 17 entities Detail

Experiments performed 20 experiments Detail

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Chemical shift validation 3 contents Detail