NMR structure of IntB phage-integrase-like protein fragment 90-199 from Erwinia carotova subsp. atroseptica: Northeast Structural Genomics Consortium target EwR217E
KSVQEKRNNT RAFKTVAKSW FATKTTWSED YQRSVWTRLE TYLFPDIGNK DIAELDTGDL LVPIKKIEKL GYLEIAMRVK QYATAIMRYA VQQKMIRFNP AYDLEGAVQK LEHHHHHH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 85.6 % (1246 of 1456) | 81.4 % (619 of 760) | 88.9 % (505 of 568) | 95.3 % (122 of 128) |
Backbone | 92.6 % (650 of 702) | 87.3 % (207 of 237) | 95.1 % (333 of 350) | 95.7 % (110 of 115) |
Sidechain | 81.2 % (705 of 868) | 78.6 % (411 of 523) | 84.9 % (282 of 332) | 92.3 % (12 of 13) |
Aromatic | 59.5 % (88 of 148) | 59.5 % (44 of 74) | 57.7 % (41 of 71) | 100.0 % (3 of 3) |
Methyl | 100.0 % (130 of 130) | 100.0 % (65 of 65) | 100.0 % (65 of 65) |
1. fragment 90-199
KSVQEKRNNT RAFKTVAKSW FATKTTWSED YQRSVWTRLE TYLFPDIGNK DIAELDTGDL LVPIKKIEKL GYLEIAMRVK QYATAIMRYA VQQKMIRFNP AYDLEGAVQK LEHHHHHHSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity | [U-99% 13C; U-99% 15N] | 1 mM | |
2 | MES | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 200 mM | |
4 | calcium chloride | natural abundance | 5 mM | |
5 | sodium azide | natural abundance | 0.02 w/v | |
6 | H2O | natural abundance | 95 % | |
7 | D2O | [U-99% 2H] | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details protein expressed in cells grown on 5% U-13C glucose, 95% unlabeled (natural abundance 13C) glucose
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | entity | [5% biosynthetically directed 13C; U-99% 15N] | 0.7 mM | |
9 | MES | natural abundance | 20 mM | |
10 | sodium chloride | natural abundance | 200 mM | |
11 | calcium chloride | natural abundance | 5 mM | |
12 | sodium azide | natural abundance | 0.02 w/v | |
13 | H2O | natural abundance | 95 % | |
14 | D2O | [U-99% 2H] | 5 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | entity | [U-99% 13C; U-99% 15N] | 0.7 mM | |
16 | MES | natural abundance | 20 mM | |
17 | sodium chloride | natural abundance | 200 mM | |
18 | calcium chloride | natural abundance | 5 mM | |
19 | sodium azide | natural abundance | 0.02 w/v | |
20 | D2O | [U-99% 2H] | 100 % |