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BMRB: 16362

2L38

1H, 13C and 15N NMR assignments of StnII-R29Q, a defective lipid binding mutant of the sea anemone actinoporin Sticholysin II.

Authors

Castrillo, I., Bruix, M., Santoro, J.

Assembly

monomer

Entity

1. monomer (polymer, Thiol state: not present), 175 monomers, 19254.60 Da Detail

ALAGTIIAGA SLTFQVLDKV LEELGKVSQK IAVGIDNESG GTWTALNAYF RSGTTDVILP EFVPNTKALL YSGRKDTGPV ATGAVAAFAY YMSSGNTLGV MFSVPFDYNW YSNWWDVKIY SGKRRADQGM YEDLYYGNPY RGDNGWHEKN LGYGLRMKGI MTSAGEAKMQ IKISR

Formula weight

19254.6 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 80.5 %, Completeness (bb): 83.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.5 % (1617 of 2008)	93.3 % (963 of 1032)	60.0 % (473 of 788)	96.3 % (181 of 188)
Backbone	83.1 % (864 of 1040)	96.4 % (353 of 366)	68.5 % (345 of 504)	97.6 % (166 of 170)
Sidechain	79.4 % (891 of 1122)	91.6 % (610 of 666)	60.7 % (266 of 438)	83.3 % (15 of 18)
Aromatic	50.0 % (110 of 220)	91.8 % (101 of 110)	4.8 % (5 of 105)	80.0 % (4 of 5)
Methyl	84.9 % (158 of 186)	100.0 % (93 of 93)	69.9 % (65 of 93)

1. R29Q StnII

ALAGTIIAGA SLTFQVLDKV LEELGKVSQK IAVGIDNESG GTWTALNAYF RSGTTDVILP EFVPNTKALL YSGRKDTGPV ATGAVAAFAY YMSSGNTLGV MFSVPFDYNW YSNWWDVKIY SGKRRADQGM YEDLYYGNPY RGDNGWHEKN LGYGLRMKGI MTSAGEAKMQ IKISR

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 4

#	Name	Isotope labeling	Concentration
1	R29Q StnII	[U-13C]; [U-15N]	0.5 (±0.1) mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 4

#	Name	Isotope labeling	Type	Concentration
4	R29Q StnII	natural abundance		0.5 (±0.1) mM
5	D2O	natural abundance		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 4 Detail

Release date

2009-11-15

Citation

(1)H, (13)C, and (15)N NMR assignments of StnII-R29Q, a defective lipid binding mutant of the sea anemone actinoporin Sticholysin II
Castrillo, I., Alegre-Cebollada, J., Martinez-Del-Pozo, A., Gavilanes, J.G., Bruix, M.
Biomol. NMR Assign. (2009), 3, 239-241, PubMed 19768581 , DOI 10.1007/s12104-009-9184-2 , Abstract

Related entities 1. monomer, : 1 : 4 : 38 entities Detail

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC