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BMRB: 16379

5UC6

Solution structure of Interleukin 1a

Authors

Mohan, S.K., Chang, H., Chin, Y.

Assembly

human interleukin 1alpha

Entity

1. human interleukin 1alpha (polymer, Thiol state: all free), 151 monomers, 17210.40 Da Detail

NVKYNFMRII KYEFILNDAL NQSIIRANDQ YLTAAALHNL DEAVKFDMGA YKSSKDDAKI TVILRISKTQ LYVTAQDEDQ PVLLKEMPEI PKTITGSETN LLFFWETHGT KNYFTSVAHP NLFIATKQDY WVCLAGGPPS ITDFQILENQ A

Formula weight

17210.4 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.4 %, Completeness: 73.1 %, Completeness (bb): 92.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	73.1 % (1326 of 1813)	72.0 % (674 of 936)	72.8 % (518 of 712)	81.2 % (134 of 165)
Backbone	92.5 % (827 of 894)	92.4 % (278 of 301)	92.9 % (416 of 448)	91.7 % (133 of 145)
Sidechain	59.9 % (638 of 1065)	62.5 % (397 of 635)	58.5 % (240 of 410)	5.0 % (1 of 20)
Aromatic	4.7 % (8 of 172)	5.8 % (5 of 86)	3.6 % (3 of 84)	0.0 % (0 of 2)
Methyl	61.3 % (114 of 186)	67.7 % (63 of 93)	54.8 % (51 of 93)

1. human interleukin 1alpha

NVKYNFMRII KYEFILNDAL NQSIIRANDQ YLTAAALHNL DEAVKFDMGA YKSSKDDAKI TVILRISKTQ LYVTAQDEDQ PVLLKEMPEI PKTITGSETN LLFFWETHGT KNYFTSVAHP NLFIATKQDY WVCLAGGPPS ITDFQILENQ A

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	10 mM
2	sodium chloride	natural abundance	100 mM
3	human interleukin 1alpha	natural abundance	1.4 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbon	internal	direct	1.0
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	nitrogen	internal	direct	1.0

Referencing offset: -0.59 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbon	internal	direct	1.0
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	nitrogen	internal	direct	1.0

Referencing offset: -0.59 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbon	internal	direct	1.0
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	nitrogen	internal	direct	1.0

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbon	internal	direct	1.0
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	nitrogen	internal	direct	1.0

Referencing offset: 0.81 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 14 models in PDB: 2KKI, Strand ID: A Detail

Release date

2009-06-29

Citation

1H, 13C and 15N backbone and side chain resonance assignments of human interleukin 1alpha
Chang, H., Mohan, S.K., Chin, Y.
Biomol. NMR Assign. (2010), 4, 59-60, PubMed 20108067 , DOI 10.1007/s12104-010-9207-z , Abstract

Related entities 1. human interleukin 1alpha, : 1 : 4 : 1 : 97 entities Detail

Interaction partners 1. human interleukin 1alpha, : 6 interactors Detail

More details for 6 interactors identified by 4 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC