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BMRB: 16402

2KKT

Solution structure of SCA7 zinc finger domain from human Ataxin-7-L3 protein

Authors

Wang, Y., Atkinson, A.R., Bonnet, J., Romier, C., Kieffer, B.

Assembly

human Ataxin-7-L3

Entity

1. SCA7 (polymer, Thiol state: all other bound), 82 monomers, 9350.439 Da Detail

HMGPEELRSL LTTQCGVISE HTKKMCTRSL RCPQHTDEQR RTVRIYFLGP SAVLPEVESS LDNDSFDMTD SQALISRLQW DG

2. ZN (non-polymer), 65.409 Da

Total weight

9415.849 Da

Max. entity weight

9350.439 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.6 %, Completeness (bb): 96.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.6 % (869 of 938)	94.5 % (463 of 490)	89.0 % (323 of 363)	97.6 % (83 of 85)
Backbone	96.5 % (467 of 484)	95.1 % (156 of 164)	97.1 % (235 of 242)	97.4 % (76 of 78)
Sidechain	90.0 % (479 of 532)	94.2 % (307 of 326)	82.9 % (165 of 199)	100.0 % (7 of 7)
Aromatic	76.9 % (40 of 52)	100.0 % (26 of 26)	52.0 % (13 of 25)	100.0 % (1 of 1)
Methyl	87.8 % (72 of 82)	95.1 % (39 of 41)	80.5 % (33 of 41)

1. SCA7

HMGPEELRSL LTTQCGVISE HTKKMCTRSL RCPQHTDEQR RTVRIYFLGP SAVLPEVESS LDNDSFDMTD SQALISRLQW DG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 (±0.4) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	50 mM
2	sodium chloride	natural abundance	200 mM
3	DTT	natural abundance	2 mM
4	entity_1	[U-99% 13C; U-99% 15N]	0.3 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 (±0.4) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
7	sodium phosphate	natural abundance	50 mM
8	sodium chloride	natural abundance	200 mM
9	DTT	natural abundance	2 mM
10	entity_1	natural abundance	0.6 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	external	direct	1.0

Referencing offset: 0.06 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	external	direct	1.0

Referencing offset: 0.06 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	external	direct	1.0

Referencing offset: -0.03 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	external	direct	1.0

Referencing offset: 0.12 ppm, Outliers: 1 Detail

Release date

2010-10-17

Citation

The structural plasticity of SCA7 domains defines their differential nucleosome-binding properties
Bonnet, J., Wang, Y., Spedale, G., Atkinson, R., Romier, C., Hamiche, A., Pijnappel, W., Timmers, H., Tora, L., Devys, D., Kieffer, B.
EMBO Rep. (2010), 11, 612-618, PubMed 20634802 , DOI 10.1038/embor.2010.98 , Abstract

Related entities 1. SCA7, : 1 : 7 entities Detail

Interaction partners 1. SCA7, : 6 interactors Detail

More details for 6 interactors identified by 2 citations

Experiments performed 7 experiments Detail

1 3D HNCO