Solution Structure of the Tandem UIM Domain of Ataxin-3 Complexed with Ubiquitin
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 72.4 % (369 of 510) | 71.9 % (194 of 270) | 70.9 % (134 of 189) | 80.4 % (41 of 51) |
Backbone | 75.7 % (209 of 276) | 87.2 % (82 of 94) | 63.2 % (86 of 136) | 89.1 % (41 of 46) |
Sidechain | 72.3 % (201 of 278) | 63.6 % (112 of 176) | 91.8 % (89 of 97) | 0.0 % (0 of 5) |
Methyl | 93.2 % (41 of 44) | 90.9 % (20 of 22) | 95.5 % (21 of 22) |
1. Ataxin-3/Ubiquitin complex
GSLDEDEEDL QRALALSRQE IDMEDEEADL RRAIQLSMQG SSRNLESolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity | [U-100% 15N] | 1 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 100 mM | |
4 | sodium azide | natural abundance | 0.02 % | |
5 | ubiquitin | natural abundance | 4 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | entity | [U-100% 13C; U-100% 15N] | 1 mM | |
7 | sodium phosphate | natural abundance | 20 mM | |
8 | sodium chloride | natural abundance | 100 mM | |
9 | sodium azide | natural abundance | 0.02 % | |
10 | ubiquitin | natural abundance | 4 mM |