Interdomain RRM packing contributes to RNA recognition in the rna15, hrp1, anchor RNA 3' processing ternary complex
KESCKMFIGG LNWDTTEDNL REYFGKYGTV TDLKIMKDPA TGRSRGFGFL SFEKPSSVDE VVKTQHILDG KVIDPKRAIP RDEQDKTGKI FVGGIGPDVR PKEFEEFFSQ WGTIIDAQLM LDKDTGQSRG FGFVTYDSAD AVDRVCQNKF IDFKDRKIEI KRAEPRH
Polymer type: polypeptide(L) polyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 27.6 % (873 of 3166) | 29.0 % (482 of 1662) | 24.0 % (297 of 1238) | 35.3 % (94 of 266) |
Backbone | 30.3 % (493 of 1625) | 29.4 % (174 of 591) | 29.6 % (235 of 795) | 35.1 % (84 of 239) |
Sidechain | 25.6 % (452 of 1769) | 28.9 % (309 of 1071) | 19.8 % (133 of 671) | 37.0 % (10 of 27) |
Aromatic | 10.0 % (33 of 330) | 20.0 % (33 of 165) | 0.0 % (0 of 157) | 0.0 % (0 of 8) |
Methyl | 33.2 % (75 of 226) | 36.3 % (41 of 113) | 30.1 % (34 of 113) |
1. rna15p
NPPSRVVYLG SIPYDQTEEQ ILDLCSNVGP VINLKMMFDP QTGRSKGYAF IEFRDLESSA SAVRNLNGYQ LGSRFLKCGY SSNS2. anchor RNA
UAUAUAUAAU AAU3. hrp1p
KESCKMFIGG LNWDTTEDNL REYFGKYGTV TDLKIMKDPA TGRSRGFGFL SFEKPSSVDE VVKTQHILDG KVIDPKRAIP RDEQDKTGKI FVGGIGPDVR PKEFEEFFSQ WGTIIDAQLM LDKDTGQSRG FGFVTYDSAD AVDRVCQNKF IDFKDRKIEI KRAEPRHSolvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | rna15p | [U-100% 15N] | 0.4 (±0.04) mM | |
2 | hrp1p | natural abundance | 0.4 (±0.04) mM | |
3 | anchor RNA | natural abundance | 0.4 (±0.04) mM | |
4 | H2O | natural abundance | 93 % | |
5 | D2O | natural abundance | 7 % | |
6 | NaCl | natural abundance | 150 mM | |
7 | Phosphate | natural abundance | 20 mM |
Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | rna15p | natural abundance | 0.6 (±0.02) mM | |
9 | hrp1p | [U-100% 15N] | 0.6 (±0.07) mM | |
10 | anchor RNA | natural abundance | 0.6 (±0.03) mM | |
11 | H2O | natural abundance | 93 % | |
12 | D2O | natural abundance | 7 % | |
13 | NaCl | natural abundance | 150 mM | |
14 | Phosphate | natural abundance | 20 mM |
Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | rna15p | [U-100% 13C] | 0.35 (±0.03) mM | |
16 | hrp1p | [U-100% 15N] | 0.35 (±0.03) mM | |
17 | anchor RNA | natural abundance | 0.35 (±0.03) mM | |
18 | H2O | natural abundance | 93 % | |
19 | D2O | natural abundance | 7 % | |
20 | NaCl | natural abundance | 150 mM | |
21 | Phosphate | natural abundance | 20 mM |
Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
22 | rna15p | [U-100% 15N] | 0.5 (±0.05) mM | |
23 | hrp1p | [U-100% 13C] | 0.5 (±0.05) mM | |
24 | anchor RNA | natural abundance | 0.5 (±0.05) mM | |
25 | H2O | natural abundance | 93 % | |
26 | D2O | natural abundance | 7 % | |
27 | NaCl | natural abundance | 150 mM | |
28 | Phosphate | natural abundance | 20 mM |
Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
29 | rna15p | [U-100% 13C; U-100% 15N] | 0.5 (±0.04) mM | |
30 | hrp1p | natural abundance | 0.5 (±0.04) mM | |
31 | anchor RNA | natural abundance | 0.5 (±0.04) mM | |
32 | H2O | natural abundance | 93 % | |
33 | D2O | natural abundance | 7 % | |
34 | NaCl | natural abundance | 150 mM | |
35 | Phosphate | natural abundance | 20 mM |
Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
36 | rna15p | natural abundance | 0.5 (±0.02) mM | |
37 | hrp1p | [U-100% 13C; U-100% 15N] | 0.5 (±0.02) mM | |
38 | anchor RNA | natural abundance | 0.5 (±0.02) mM | |
39 | H2O | natural abundance | 93 % | |
40 | D2O | natural abundance | 7 % | |
41 | NaCl | natural abundance | 150 mM | |
42 | Phosphate | natural abundance | 20 mM |