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BMRB: 16442

2KMW

Solution structure of At3g03773.1 protein from Arabidopsis thaliana

Authors

Sahu, S.C., Singh, S., Tonelli, M., Markley, J.L.

Assembly

At3g03773.1

Entity

1. At3g03773.1 (polymer, Thiol state: all free), 150 monomers, 17322.00 Da Detail

SSRNPEVLWA QRSDKVYLTV ALPDAKDISV KCEPQGLFSF SALGAQGERF EFSLELYGKI MTEYRKNVGL RNIIFSIQKE ERSWWTRLLK SEEKPAPYIK VDWNKWCDED EEVNSETASD DESAFVNQDS ESSDDDGLLY LPDLEKARNK

Formula weight

17322.0 Da

Source organism

Arabidopsis thaliana

Exptl. method

solution NMR

Refine. method

simulated annealing, distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.0 %, Completeness (bb): 97.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.0 % (1710 of 1782)	95.7 % (894 of 934)	98.1 % (674 of 687)	88.2 % (142 of 161)
Backbone	97.3 % (864 of 888)	97.3 % (292 of 300)	97.3 % (432 of 444)	97.2 % (140 of 144)
Sidechain	95.3 % (989 of 1038)	95.0 % (602 of 634)	99.5 % (385 of 387)	11.8 % (2 of 17)
Aromatic	97.5 % (156 of 160)	100.0 % (80 of 80)	100.0 % (75 of 75)	20.0 % (1 of 5)
Methyl	100.0 % (138 of 138)	100.0 % (69 of 69)	100.0 % (69 of 69)

1. At3g03773.1

SSRNPEVLWA QRSDKVYLTV ALPDAKDISV KCEPQGLFSF SALGAQGERF EFSLELYGKI MTEYRKNVGL RNIIFSIQKE ERSWWTRLLK SEEKPAPYIK VDWNKWCDED EEVNSETASD DESAFVNQDS ESSDDDGLLY LPDLEKARNK

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	entity	[U-13C; U-15N]	1 (±0.1) mM
2	H2O	natural abundance	93 %
3	H2O	natural abundance	7 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.33 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.33 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.38 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KMW, Strand ID: A Detail

Release date

2010-09-02

Citation

Solution structure of At3g03773.1 protein from Arabidopsis thaliana
Sahu, S.C., Singh, S., Tonelli, M., Markley, J.L.
Structure

Related entities 1. At3g03773.1, : 1 : 2 : 28 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC