BMRB: 16454

NMR Solution Structure of Complement-like Repeat CR3 from the Low Density Lipoprotein REceptor-related Protein

Authors

Dolmer, K., Huang, W., Gettins, P.G.W.

Assembly

CR3/RBD

Entity

1. CR3 (polymer, Thiol state: not reported), 41 monomers, 4608.951 Da Detail

QCQPGEFACA NSRCIQERWK CDGDNDCLDN SDEAPALCHQ H

2. RBD (polymer, Thiol state: not reported), 357 monomers, 41552.51 Da Detail

MAPRRVRSFL RGLPALLLLL LFLGPWPAAS HGGKYSREKN QPKPSPKRES GEEFRMEKLN QLWEKAQRLH LPPWRLAELH ADLKIQERDE LAWKKLKLDG LDEDGEKEAR LIRNLNVILA KYGLDGKKDA RQVTSNSLSG TQEDGLDDPR LEKLWHKAKT SGKFSGEELD KLWREFLHHK EKVHEYNVLL ETLSRTEEIH ENVISPSDLS DIKGSVLHSR HTELKEKLRS INQGLDRLRR VSHQGYSTEA EFEEPRVIDL WDLAQSANLT DKELEAFREE LKHFEAKIEK HNHYQKQLEI AHEKLRHAES VGDGERVSRS REKHALLEGR TKELGYTVKK HLQDLSGRIS RARHNEL

3. CA (non-polymer, Thiol state: not present), 40.078 Da

Total weight

46201.54 Da

Max. entity weight

41552.51 Da

Source organism

Pseudomonas

Exptl. method

solution NMR

Data set

binding_constants

Binding constant

1 Kd values in 1 lists
Temperature 298 K, pH 7.4 Detail

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List #1 binding_data, Participants 1. CR3, 2. RBD,

Sample ID	Exp. name	Binding reaction	Obs. entity ID	Obs. parameter	Value type	Value	Value error
1	2D 1H-15N HSQC	1 + 2 ⇄ 12	1	chemical shifts	Kd	140 uM

Release date

2010-03-07

Citation

NMR solution structure of complement-like repeat CR3 from the low density lipoprotein receptor-related protein. Evidence for specific binding to the receptor binding domain of human alpha(2)-macroglobulin
Dolmer, K., Huang, W., Gettins, P.G.W.
J. Biol. Chem. (2000), 275, 3264-3269, PubMed 10652313 , DOI 10.2210/pdb1d2l/pdb , Abstract

Related entities 1. CR3, : 1 : 1 : 537 entities Detail

Related entities 2. RBD, : 1 : 3 : 6 entities Detail

Interaction partners 1. CR3, : 22 : 1 interactors Detail

Interaction partners 2. RBD, : 16 : 1 interactors Detail

Experiments performed 1 experiments Detail