BMRB: 16456

The layered fold of the TSR domain of P. falciparum TRAP contains a heparin binding site

Authors

Tossavainen, H., Pihalajamaa, T., Huttenen, T.K., Raulo, E., Rauvala, H., Permi, P., Kilperlainen, I.

Assembly

TRAP-TSR/heparin

Entity

1. TRAP-TSR (polymer, Thiol state: not reported), 49 monomers, 5442.964 Da Detail

GSASCGVWDE WSPCSVTCGK GTRSRKREIL HEGCTSEIQE QCEEERCPP

2. heparin (polymer, Thiol state: not reported), 225 monomers, 24276.24 Da Detail

IVGGRKARPR QFPFLASIQN QGRHFCGGAL IHARFVMTAA SCFQSQNPGV STVVLGAYDL RRRERQSRQT FSISSMSENG YDPQQNLNDL MLLQLDREAN LTSSVTILPL PLQNATVEAG TRCQVAGWGS QRSGGRLSRF PRFVNVTVTP EDQCRPNNVC TGVLTRRGGI CNGDGGTPLV CEGLAHGVAS FSLGPCGRGP DFFTRVALFR DWIDGVLNNP GPGPA

Total weight

29719.203 Da

Max. entity weight

24276.24 Da

Source organism

Escherichia coli , Homo sapiens

Exptl. method

solution NMR

Data set

binding_constants

Binding constant

8 Kd values in 1 lists
Pressure 1 atm, Temperature 283 K, pH 6.6 Detail

---

List #1 binding_data, Participants 1. TRAP-TSR, 2. heparin,

Sample ID	Exp. name	Binding reaction	Obs. entity ID	Obs. parameter	Value type	Value	Value error
1	2D 1H-15N HSQC	1 + 2 ⇄ 12	1	chemical shifts	Kd	0.2 mM
2	2D 1H-15N HSQC	1 + 2 ⇄ 12	1	chemical shifts	Kd	0.2 mM
3	2D 1H-15N HSQC	1 + 2 ⇄ 12	1	chemical shifts	Kd	0.2 mM
4	2D 1H-15N HSQC	1 + 2 ⇄ 12	1	chemical shifts	Kd	0.2 mM
5	2D 1H-15N HSQC	1 + 2 ⇄ 12	1	chemical shifts	Kd	0.2 mM
6	2D 1H-15N HSQC	1 + 2 ⇄ 12	1	chemical shifts	Kd	0.2 mM
7	2D 1H-15N HSQC	1 + 2 ⇄ 12	1	chemical shifts	Kd	0.2 mM
8	2D 1H-15N HSQC	1 + 2 ⇄ 12	1	chemical shifts	Kd	0.2 mM

Release date

2010-03-07

Citation

The layered fold of the TSR domain of P. falciparum TRAP contains a heparin binding site
Tossavainen, H., Pihalajamaa, T., Huttenen, T.K., Raulo, E., Rauvala, H., Permi, P., Kilperlainen, I.
Protein Sci. (2006), 15, 1760-1768, PubMed 16815922 , DOI 10.1110/ps.052068506 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. TRAP-TSR, : 1 : 146 entities Detail

Related entities 2. heparin, : 1 : 99 entities Detail

Experiments performed 8 experiments Detail