BMRB: 16459

Solution Structure of the Ran-binding Domain 2 of RanBP and its Interaction with the C Terminus of Ran

Authors

Geyer, J.P., Doker, R.D., Kremer, W.D., Zhao, X., Kuhlmann, J., Kalbitzer, H.R.

Assembly

Ran BD2/Ran C complex

Entity

1. RanBD2(delta)N (polymer, Thiol state: not reported), 130 monomers, 15115.28 Da Detail

GSGEEDEKVL YSQRVKLFRF DAEVSQWKER GLGNLKILKN EVNGKLRMLM RREQVLKVCA NHWITTTMNL KPLSGSDRAW MWLASDFSDG DAKLEQLAAK FKTPELAEEF KQKFEECQRL LLDIPLQTPK

2. peptide 2 (polymer, Thiol state: not present), 28 monomers, 3087.235 Da Detail

MDPALAAQYE HDLEVAQTTA LPDEDDDL

Total weight

18202.516 Da

Max. entity weight

15115.28 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Simulated annealing

Data set

binding_constants

Protein Blocks Logo

Calculated from 20 models in PDB: 1XKE, Strand ID: A Detail

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Binding constant

2 Kd values in 1 lists
Pressure 1 atm, Temperature 298 K, pH 6.5 Detail

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List #1 binding_data, Participants 1. RanBD2(delta)N, 2. peptide 2,

Sample ID	Exp. name	Binding reaction	Obs. entity ID	Obs. parameter	Value type	Value	Value error
1	2D 1H-15N TROSY	1 + 2 ⇄ 12	1	chemical shifts	Kd	130 uM	96 uM
1	2D 1H-15N HSQC	1 + 2 ⇄ 12	1	chemical shifts	Kd	130 uM	96 uM

Release date

2010-03-07

Citation

Solution structure of the Ran-binding domain 2 of RanBP2 and its interaction with the C terminus of Ran
Geyer, J.P., Doker, R., Kremer, W., Zhao, X., Kuhlmann, J., Kalbitzer, H.R.
J. Mol. Biol. (2005), 348, 711-725, PubMed 15826666 , DOI 10.1016/j.jmb.2005.02.033 , Abstract

Related entities 1. RanBD2(delta)N, : 52 entities Detail

Related entities 2. peptide 2, : 1 : 40 : 4 : 2 : 23 entities Detail

Interaction partners 2. peptide 2, : 1 interactors Detail

Experiments performed 2 experiments Detail