Solution structure of zinc-substituted rubredoxin B (Rv3250c) from Mycobacterium tuberculosis. Seattle Structural Genomics Center for Infectious Disease(SSGCID)target MytuD.01635a
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS30:SG | 2:ZN1:ZN |
2 | na | sing | 1:CYS33:SG | 2:ZN1:ZN |
3 | na | sing | 1:CYS63:SG | 2:ZN1:ZN |
4 | na | sing | 1:CYS66:SG | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 78.7 % (700 of 889) | 77.9 % (357 of 458) | 78.4 % (272 of 347) | 84.5 % (71 of 84) |
Backbone | 84.1 % (402 of 478) | 85.5 % (142 of 166) | 82.1 % (193 of 235) | 87.0 % (67 of 77) |
Sidechain | 74.4 % (360 of 484) | 73.6 % (215 of 292) | 76.2 % (141 of 185) | 57.1 % (4 of 7) |
Aromatic | 69.8 % (74 of 106) | 69.8 % (37 of 53) | 68.0 % (34 of 50) | 100.0 % (3 of 3) |
Methyl | 77.8 % (42 of 54) | 77.8 % (21 of 27) | 77.8 % (21 of 27) |
1. Rv3250c
MAHHHHHHMG TLEAQTQGPG SMNDYKLFRC IQCGFEYDEA LGWPEDGIAA GTRWDDIPDD WSCPDCGAAK SDFEMVEVAR SSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.1 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | sodium chloride | natural abundance | 100 (±2.0) mM | |
2 | DTT | natural abundance | 1 (±0.05) mM | |
3 | TRIS | natural abundance | 20 (±0.2) mM | |
4 | Rv3250c | [U-98% 13C; U-98% 15N] | 1-2 (±0.1) mM | |
5 | D2O | natural abundance | 10 % | |
6 | H2O | natural abundance | 90 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.1 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | sodium chloride | natural abundance | 100 (±2.0) mM | |
8 | DTT | natural abundance | 1 (±0.05) mM | |
9 | TRIS | natural abundance | 20 (±0.2) mM | |
10 | Rv3250c | [U-98% 13C; U-98% 15N] | 1-2 (±0.1) mM | |
11 | D2O | natural abundance | 100 % |