BMRBj - BMREntryMaster

Found 1 Document (0.723 seconds)

BMRB: 16542

3EIQ

15N, 13C and 1H Backbone Resonance Assignments for the Complete Tandem MA-3 Region of the Tumour Suppressor Protein Pdcd4

Authors

Waters, L.C., Oka, O., Muskett, F.W., Strong, S.L., Schmedt, T., Klempnauer, K., Carr, M.D.

Assembly

Pdcd4 MA-3 M-C

Entity

1. Pdcd4 MA-3 M-C (polymer, Thiol state: all free), 298 monomers, 33001.50 Da Detail

GPLGSLPLDE TAFEKTLTPI IQEYFEHGDT NEVAEMLRDL NLGEMKSGVP VLAVSLALEG KASHREMTSK LLSDLCGTVM STNDVEKSFD KLLKDLPELA LDTPRAPQLV GQFIARAVGD GILCNTYIDS YKGTVDCVQA RAALDKATVL LSMSKGGKRK DSVWGSGGGQ QPVNHLVKEI DMLLKEYLLS GDISEAEHCL KELEVPHFHH ELVYEAIVMV LESTGESAFK MILDLLKSLW KSSTITIDQM KRGYERIYNE IPDINLDVPH SYSVLERFVE ECFQAGIISK QLRDLCPS

Formula weight

33001.5 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.3 %, Completeness: 42.1 %, Completeness (bb): 72.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	42.1 % (1445 of 3434)	25.6 % (456 of 1778)	52.8 % (714 of 1352)	90.5 % (275 of 304)
Backbone	72.4 % (1278 of 1764)	53.7 % (325 of 605)	77.9 % (680 of 873)	95.5 % (273 of 286)
Sidechain	14.9 % (291 of 1947)	11.2 % (131 of 1173)	20.9 % (158 of 756)	11.1 % (2 of 18)
Aromatic	1.5 % (3 of 200)	2.0 % (2 of 100)	1.0 % (1 of 98)	0.0 % (0 of 2)
Methyl	15.9 % (59 of 370)	15.1 % (28 of 185)	16.8 % (31 of 185)

1. Pdcd4 MA-3 M-C

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Pdcd4 MA-3 M-C	[U-99% 13C; U-99% 15N]	0.15-0.5 mM
2	Sodium Phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	2.5 mM
5	EDTA	natural abundance	50 uM
6	AEBSF protease inhibitor	natural abundance	200 uM
7	Sodium Azide	natural abundance	0.02 %
8	TCEP	natural abundance	2.5 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	Pdcd4 MA-3 M-C	[U-99% 15N]	0.5 mM
10	Sodium Phosphate	natural abundance	25 mM
11	sodium chloride	natural abundance	100 mM
12	DTT	natural abundance	2.5 mM
13	EDTA	natural abundance	50 uM
14	AEBSF protease inhibitor	natural abundance	200 uM
15	Sodium Azide	natural abundance	0.02 %
16	TCEP	natural abundance	2.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.21 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.21 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

Release date

2010-01-11

Citation

Resonance assignment and secondary structure of the middle MA-3 domain and complete tandem MA-3 region of the tumour suppressor protein Pdcd4
Waters, L.C., Oka, O., Muskett, F.W., Strong, S.L., Schmedt, T., Klempnauer, K., Carr, M.D.
Biomol. NMR Assign. (2010), 4, 49-53, PubMed 20020227 , DOI 10.1007/s12104-009-9205-1 , Abstract

Related entities 1. Pdcd4 MA-3 M-C, : 1 : 2 : 51 entities Detail

Interaction partners 1. Pdcd4 MA-3 M-C, : 6 interactors Detail

More details for 6 interactors identified by 5 citations

Experiments performed 6 experiments Detail

1 3D CBCA(CO)NH