15N, 13C and 1H Backbone Resonance Assignments for the Complete Tandem MA-3 Region of the Tumour Suppressor Protein Pdcd4
GPLGSLPLDE TAFEKTLTPI IQEYFEHGDT NEVAEMLRDL NLGEMKSGVP VLAVSLALEG KASHREMTSK LLSDLCGTVM STNDVEKSFD KLLKDLPELA LDTPRAPQLV GQFIARAVGD GILCNTYIDS YKGTVDCVQA RAALDKATVL LSMSKGGKRK DSVWGSGGGQ QPVNHLVKEI DMLLKEYLLS GDISEAEHCL KELEVPHFHH ELVYEAIVMV LESTGESAFK MILDLLKSLW KSSTITIDQM KRGYERIYNE IPDINLDVPH SYSVLERFVE ECFQAGIISK QLRDLCPS
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 42.1 % (1445 of 3434) | 25.6 % (456 of 1778) | 52.8 % (714 of 1352) | 90.5 % (275 of 304) |
Backbone | 72.4 % (1278 of 1764) | 53.7 % (325 of 605) | 77.9 % (680 of 873) | 95.5 % (273 of 286) |
Sidechain | 14.9 % (291 of 1947) | 11.2 % (131 of 1173) | 20.9 % (158 of 756) | 11.1 % (2 of 18) |
Aromatic | 1.5 % (3 of 200) | 2.0 % (2 of 100) | 1.0 % (1 of 98) | 0.0 % (0 of 2) |
Methyl | 15.9 % (59 of 370) | 15.1 % (28 of 185) | 16.8 % (31 of 185) |
1. Pdcd4 MA-3 M-C
GPLGSLPLDE TAFEKTLTPI IQEYFEHGDT NEVAEMLRDL NLGEMKSGVP VLAVSLALEG KASHREMTSK LLSDLCGTVM STNDVEKSFD KLLKDLPELA LDTPRAPQLV GQFIARAVGD GILCNTYIDS YKGTVDCVQA RAALDKATVL LSMSKGGKRK DSVWGSGGGQ QPVNHLVKEI DMLLKEYLLS GDISEAEHCL KELEVPHFHH ELVYEAIVMV LESTGESAFK MILDLLKSLW KSSTITIDQM KRGYERIYNE IPDINLDVPH SYSVLERFVE ECFQAGIISK QLRDLCPSSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Pdcd4 MA-3 M-C | [U-99% 13C; U-99% 15N] | 0.15-0.5 mM | |
2 | Sodium Phosphate | natural abundance | 25 mM | |
3 | sodium chloride | natural abundance | 100 mM | |
4 | DTT | natural abundance | 2.5 mM | |
5 | EDTA | natural abundance | 50 uM | |
6 | AEBSF protease inhibitor | natural abundance | 200 uM | |
7 | Sodium Azide | natural abundance | 0.02 % | |
8 | TCEP | natural abundance | 2.5 mM |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | Pdcd4 MA-3 M-C | [U-99% 15N] | 0.5 mM | |
10 | Sodium Phosphate | natural abundance | 25 mM | |
11 | sodium chloride | natural abundance | 100 mM | |
12 | DTT | natural abundance | 2.5 mM | |
13 | EDTA | natural abundance | 50 uM | |
14 | AEBSF protease inhibitor | natural abundance | 200 uM | |
15 | Sodium Azide | natural abundance | 0.02 % | |
16 | TCEP | natural abundance | 2.5 mM |