Solution NMR structure of the Mus81 N-terminal HhH. Northeast Structural Genomics Consortium target MmT1A
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 80.4 % (793 of 986) | 86.7 % (456 of 526) | 69.5 % (264 of 380) | 91.3 % (73 of 80) |
Backbone | 79.6 % (371 of 466) | 93.7 % (148 of 158) | 66.0 % (155 of 235) | 93.2 % (68 of 73) |
Sidechain | 82.7 % (492 of 595) | 83.7 % (308 of 368) | 81.4 % (179 of 220) | 71.4 % (5 of 7) |
Aromatic | 47.6 % (40 of 84) | 54.8 % (23 of 42) | 37.5 % (15 of 40) | 100.0 % (2 of 2) |
Methyl | 89.2 % (66 of 74) | 89.2 % (33 of 37) | 89.2 % (33 of 37) |
1. Mus81 NTD
RPLPVCPNPP FVRWLTEWRD EAASRGRHTR FVFQKALRSL QRYPLPLRSG KEAKILQHFG DRLCRMLDEK LKQHLASGGDSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Mus81 NTD | [U-100% 13C; U-100% 15N] | 1.0 mM | |
2 | sodium phosphate | natural abundance | 25 mM | |
3 | sodium chloride | natural abundance | 500 mM | |
4 | DTT | natural abundance | 2 mM | |
5 | benzamidine | natural abundance | 2 mM | |
6 | PMSF | natural abundance | 0.5 mM | |
7 | EDTA | natural abundance | 0.5 mM | |
8 | TCEP | natural abundance | 0.5 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | Mus81 NTD | [U-100% 13C; U-100% 15N] | 1 mM | |
10 | sodium phosphate | natural abundance | 25 mM | |
11 | sodium chloride | natural abundance | 500 mM | |
12 | benzamidine | natural abundance | 2 mM | |
13 | DTT | natural abundance | 2 mM | |
14 | EDTA | natural abundance | 0.5 mM | |
15 | PMSF | natural abundance | 0.5 mM | |
16 | TCEP | natural abundance | 0.5 mM |