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BMRB: 16567

2KVI

1H, 13C, and 15N chemical shift assignments for the RNA recognition motif of Nab3

Authors

Stefl, R., Kubicek, K., Pergoli, R., Hobor, F., Pasulka, J.

Assembly

Nab3 RRM

Entity

1. Nab3 RRM (polymer, Thiol state: all free), 104 monomers, 12043.45 Da Detail

MTEMHNIPPK SRLFIGNLPL KNVSKEDLFR IFSPYGHIMQ INIKNAFGFI QFDNPQSVRD AIECESQEMN FGKKLILEVS SSNARPQFDH GDHGTNLEHH HHHH

Formula weight

12043.45 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 65.4 %, Completeness: 48.0 %, Completeness (bb): 51.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	48.0 % (603 of 1256)	50.2 % (331 of 660)	43.4 % (210 of 484)	55.4 % (62 of 112)
Backbone	51.6 % (316 of 612)	58.2 % (121 of 208)	43.8 % (134 of 306)	62.2 % (61 of 98)
Sidechain	47.3 % (351 of 742)	46.5 % (210 of 452)	50.7 % (140 of 276)	7.1 % (1 of 14)
Aromatic	14.1 % (18 of 128)	14.1 % (9 of 64)	14.1 % (9 of 64)
Methyl	65.1 % (56 of 86)	65.1 % (28 of 43)	65.1 % (28 of 43)

1. RNA binding polypeptide

MTEMHNIPPK SRLFIGNLPL KNVSKEDLFR IFSPYGHIMQ INIKNAFGFI QFDNPQSVRD AIECESQEMN FGKKLILEVS SSNARPQFDH GDHGTNLEHH HHHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 8

#	Name	Isotope labeling	Type	Concentration
1	RNA binding polypeptide	[U-99% 13C; U-99% 15N]		3 mM

LACS Plot; CA

Referencing offset: 1.85 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: 1.85 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

Release date

2010-05-03

Citation

1H, 13C, and 15N chemical shift assignments for the RNA recognition motif of Nab3
Pergoli, R., Kubicek, K., Hobor, F., Pasulka, J., Stefl, R.
Biomol. NMR Assign. (2010), 4, 119-121, PubMed 20309651 , DOI 10.1007/s12104-010-9221-1 , Abstract

Related entities 1. Nab3 RRM, : 1 : 50 entities Detail

Interaction partners 1. Nab3 RRM, : 33 interactors Detail

More details for 33 interactors identified by 8 citations

Experiments performed 11 experiments Detail

1 3D HNCA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 8

#	Name	Isotope labeling	Type	Concentration
1	RNA binding polypeptide	[U-99% 13C; U-99% 15N]		3 mM

2 3D HN(CO)CA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 8

#	Name	Isotope labeling	Type	Concentration
1	RNA binding polypeptide	[U-99% 13C; U-99% 15N]		3 mM

3 3D HNCACB

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 8

#	Name	Isotope labeling	Type	Concentration
1	RNA binding polypeptide	[U-99% 13C; U-99% 15N]		3 mM

4 3D CBCA(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 8

#	Name	Isotope labeling	Type	Concentration
1	RNA binding polypeptide	[U-99% 13C; U-99% 15N]		3 mM

5 3D HNHA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 8

#	Name	Isotope labeling	Type	Concentration
1	RNA binding polypeptide	[U-99% 13C; U-99% 15N]		3 mM

6 2D 1H-1H NOESY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 8

#	Name	Isotope labeling	Type	Concentration
1	RNA binding polypeptide	[U-99% 13C; U-99% 15N]		3 mM

7 3D HCCH-TOCSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 8

#	Name	Isotope labeling	Type	Concentration
1	RNA binding polypeptide	[U-99% 13C; U-99% 15N]		3 mM

8 3D 1H-15N NOESY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 8

#	Name	Isotope labeling	Type	Concentration
1	RNA binding polypeptide	[U-99% 13C; U-99% 15N]		3 mM

9 3D 1H-13C NOESY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 8

#	Name	Isotope labeling	Type	Concentration
1	RNA binding polypeptide	[U-99% 13C; U-99% 15N]		3 mM

10 2D 1H-15N HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 8

#	Name	Isotope labeling	Type	Concentration
1	RNA binding polypeptide	[U-99% 13C; U-99% 15N]		3 mM

11 2D 1H-13C HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 8

#	Name	Isotope labeling	Type	Concentration
1	RNA binding polypeptide	[U-99% 13C; U-99% 15N]		3 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr16567_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr16567_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	637		64.4 (chain: 1, length: 104)
1	Chemical shift references	chemical_shift_reference_1	OK	4		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 104, Sequence coverage: 64.4%
- Total number of assignments
  - ¹H chemical shifts: 376
  - ¹³C chemical shifts: 201
  - ¹⁵N chemical shifts: 60
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1