NMR Structure of YbbR family protein Dhaf_0833 (residues 32-118) from Desulfitobacterium hafniense DCB-2: Northeast Structural Genomics Consortium target DhR29B
SSQTLDRDPT LTLSLIAKNT PANSMIMTKL PSVRVKTEGY NPSINVNELF AYVDLSGSEP GEHDYEVKVE PIPNIKIVEI SPRVVTLQLE HHHHHH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 91.3 % (1016 of 1113) | 91.9 % (530 of 577) | 89.8 % (395 of 440) | 94.8 % (91 of 96) |
Backbone | 93.0 % (521 of 560) | 94.1 % (176 of 187) | 91.9 % (262 of 285) | 94.3 % (83 of 88) |
Sidechain | 90.2 % (583 of 646) | 90.8 % (354 of 390) | 89.1 % (221 of 248) | 100.0 % (8 of 8) |
Aromatic | 32.3 % (20 of 62) | 32.3 % (10 of 31) | 32.3 % (10 of 31) | |
Methyl | 97.5 % (117 of 120) | 96.7 % (58 of 60) | 98.3 % (59 of 60) |
1. Dhaf 0833
SSQTLDRDPT LTLSLIAKNT PANSMIMTKL PSVRVKTEGY NPSINVNELF AYVDLSGSEP GEHDYEVKVE PIPNIKIVEI SPRVVTLQLE HHHHHHSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 4.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Dhaf_0833 (32-118) | [U-100% 13C; U-100% 15N] | 0.8 mM | |
2 | ammonium acetate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 200 mM | |
4 | calcium chloride | natural abundance | 10 mM | |
5 | sodium azide | natural abundance | 0.02 % | |
6 | H2O | natural abundance | 95 % | |
7 | D2O | natural abundance | 5 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 4.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | Dhaf_0833 (32-118) | [U-100% 13C; U-100% 15N] | 0.8 mM | |
9 | ammonium acetate | natural abundance | 20 mM | |
10 | sodium chloride | natural abundance | 200 mM | |
11 | calcium chloride | natural abundance | 10 mM | |
12 | sodium azide | natural abundance | 0.02 % | |
13 | D2O | natural abundance | 100 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 4.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | Dhaf_0833 (32-118) | [5% 13C from 5% U-13C glucose in growth medium; U-100% 15N] | 0.8 mM | |
15 | ammonium acetate | natural abundance | 20 mM | |
16 | sodium chloride | natural abundance | 200 mM | |
17 | calcium chloride | natural abundance | 10 mM | |
18 | sodium azide | natural abundance | 0.02 % | |
19 | H2O | natural abundance | 95 % | |
20 | D2O | natural abundance | 5 % |