Solution NMR structure of the apo form of a ribonuclease H domain of protein DSY1790 from Desulfitobacterium hafniense, Northeast Structural Genomics target DhR1A.
MDDRTEYDVY TDGSYVNGQY AWAYAFVKDG KVHYEDADVG KNPAAATMRN VAGEIAAALY AVKKASQLGV KIRILHDYAG IAFWATGEWK AKNEFTQAYA KLMNQYRGIY SFEKVKAHSG NEFNDYVDMK AKSALGIRDL EHHHHHH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 75.1 % (1278 of 1702) | 82.5 % (721 of 874) | 63.3 % (423 of 668) | 83.7 % (134 of 160) |
Backbone | 75.1 % (661 of 880) | 87.5 % (266 of 304) | 62.1 % (267 of 430) | 87.7 % (128 of 146) |
Sidechain | 76.5 % (733 of 958) | 79.8 % (455 of 570) | 72.7 % (272 of 374) | 42.9 % (6 of 14) |
Aromatic | 61.9 % (135 of 218) | 76.1 % (83 of 109) | 46.2 % (49 of 106) | 100.0 % (3 of 3) |
Methyl | 91.5 % (130 of 142) | 91.5 % (65 of 71) | 91.5 % (65 of 71) |
1. DSY1790
MDDRTEYDVY TDGSYVNGQY AWAYAFVKDG KVHYEDADVG KNPAAATMRN VAGEIAAALY AVKKASQLGV KIRILHDYAG IAFWATGEWK AKNEFTQAYA KLMNQYRGIY SFEKVKAHSG NEFNDYVDMK AKSALGIRDL EHHHHHHSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DhR1A | [U-99% 13C; U-99% 15N] | 0.89 mM | |
2 | D2O | [U-2H] | 10 v/v | |
3 | H2O | natural abundance | 90 v/v | |
4 | MES | natural abundance | 20 mM | |
5 | sodium chloride | natural abundance | 200 mM | |
6 | calcium chloride | natural abundance | 5 mM | |
7 | DTT | natural abundance | 10 mM | |
8 | DSS | natural abundance | 50 uM | |
9 | sodium azide | natural abundance | 0.02 w/v |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | DhR1A | [U-5% 13C; U-99% 15N] | 0.943 mM | |
11 | D2O | [U-2H] | 10 v/v | |
12 | H2O | natural abundance | 90 v/v | |
13 | MES | natural abundance | 20 mM | |
14 | sodium chloride | natural abundance | 200 mM | |
15 | calcium chloride | natural abundance | 5 mM | |
16 | DTT | natural abundance | 10 mM | |
17 | DSS | natural abundance | 50 uM | |
18 | sodium azide | natural abundance | 0.02 w/v |