BMRBj - BMREntryMaster

Found 1 Document (0.72 seconds)

BMRB: 16580

4BU4

2J coupling constants in Ribonuclease T1 from Aspergillus oryzae

Authors: Schmidt, J.M., Lohr, F.
Assembly: Ribonuclease T1
Entity: 1. Ribonuclease T1 (polymer, Thiol state: all disulfide bound), 104 monomers, 11088.64 Da Detail
ACDYTCGSNC YSSSDVSTAQ AAGYKLHEDG ETVGSNSYPH KYNNYEGFDF SVSSPYYEWP ILSSGDVYSG GSPGADRVVF NENNQLAGVI THTGASGNNF VECT
Formula weight: 11088.64 Da
Source organism: Aspergillus oryzae
Exptl. method: solution NMR
Data set: coupling_constants
Coupling constant: 824 J values in 10 lists
Field strength (¹H) 950 MHz, 500 MHz, 600 MHz, 800 MHz, 900 MHz, Pressure 1 atm, Temperature 308 K, pH 5.5 Detail
Pressure 1 atm, Temperature 308 K, pH 5.5
Experiment name 2D CO-coupled ct-[13C,1H]-HSQC, 3D Ha-coupled ct-[15N,1H]-TROSY-iHNCA, 3D CO-coupled ct-[15N,1H]-TROSY-HNCA, 3D Ca-coupled [15N,1H]-TROSY-HNCO, 3D Cb-coupled [15N,1H]-TROSY-HNCA, 2D IPAP-type HN(a/b-NCO-J)-TROSY, 3D Cb-coupled [15N,1H]-TROSY-H(N)COCA, 3D Ha-coupled [15N,1H]-TROSY-H(N)COCA, 2D IPAP-type HN(CO-a/b-NCa-J)-TROSY, 3D CO-coupled (H)CANNH, 2D Cb-coupled [13C,1H]-HSQC
List #1 2JCaN_, Field strength (¹H) 600 MHz
List #2 2JHNCa, Field strength (¹H) 600 MHz
List #3 2JCOHN, Field strength (¹H) 900 MHz
List #4 2JCOCa, Field strength (¹H) 500 MHz
List #5 2JHaCO, Field strength (¹H) 600 MHz
List #6 2JHaCb, Field strength (¹H) 950 MHz
List #7 2JCbCO, Field strength (¹H) 500 MHz
List #8 2JN_HA, Field strength (¹H) 500 MHz
List #9 2JN_CB, Field strength (¹H) 800 MHz
List #10 2JN_CO, Field strength (¹H) 600 MHz
Release date: 2010-02-07
Citation 1: Correlation of (2)J couplings with protein secondary structure
Schmidt, J.M., Hua, Y., Lohr, F.
Proteins (2010), 78, 1544-1562, PubMed 20131375 , DOI 10.1002/prot.22672 , Abstract
Citation 2: Measurement of two-bond JCOH alpha coupling constants in proteins uniformly enriched with 13C
Vuister, G.W., Bax, A.
J. Biomol. NMR (1992), 2, 401-405, PubMed 1511238 , Abstract
Citation 3: (H)NCAHA and (H)CANNH experiments for the determination of the vicinal coupling constants related to the phi-torsion angle
Lohr, F., Ruterjans, H.
J. Biomol. NMR (1995), 5, 25-36, PubMed 7881271 , Abstract
Citation 4: Determination of the backbone dihedral angle in human ubiquitin from reparametrized empirical Karplus equations
Wang, A.C., Bax, A.
J. Am. Chem. Soc. (1996), 118, 2483-2494, DOI 10.1021/ja9535524
Citation 5: [13C]-constant-time [15N,1H]-TROSY-HNCA for sequential assignment of large proteins
Salzmann, M., Pervushin, K., Wider, G., Senn, H., Wuthrich, K.
J. Biomol. NMR (1999), 14, 85-88
Citation 6: Transverse relaxation optimised spin-state selective NMR experiments for measurement of residual dipolar couplings
Permi, P., Annila, A.
J. Biomol. NMR (2000), 16, 221-227, PubMed 10805128 , Abstract
Citation 7: Measurement of residual dipolar couplings from 1Halpha to 13Calpha and 15N using a simple HNCA-based experiment
Permi, P.
J. Biomol. NMR (2003), 27, 341-349, PubMed 14512731 , Abstract
Citation 8: Determination of spin-spin couplings from ultrahigh resolution 3D NMR spectra obtained by optimized random sampling and multidimensional Fourier transformation
Kazimierczuk, K., Zawadzka, A., Kozminski, W., Zhukov, I.
J. Am. Chem. Soc. (2008), 130, 5404-5405, PubMed 18376830 , DOI 10.1021/ja800622p , Abstract

Related entities 1. Ribonuclease T1, : 1 : 25 : 11 : 27 : 34 entities Detail

Experiments performed 11 experiments Detail

1 3D Ca-coupled [15N,1H]-TROSY-HNCO

Instrument

Bruker DRX - 500 MHz 1H{13C,15N}-triple-resonance three-axis PFG probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	RNase_T1	[U-95% 13C; U-95% 15N]	2 mM
2	D2O	[U-2H]	10 %
3	H2O	natural abundance	90 %

2 2D IPAP-type HN(CO-a/b-NCa-J)-TROSY

Instrument

Bruker Avance - 600 MHz room-temperature and cryogenic triple-resonance z-gradient probes

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	RNase_T1	[U-95% 13C; U-95% 15N]	2 mM
2	D2O	[U-2H]	10 %
3	H2O	natural abundance	90 %

3 2D IPAP-type HN(a/b-NCO-J)-TROSY

Instrument

Bruker Avance - 900 MHz cryogenic triple-resonance z-gradient probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	RNase_T1	[U-95% 13C; U-95% 15N]	2 mM
2	D2O	[U-2H]	10 %
3	H2O	natural abundance	90 %

4 3D Ha-coupled ct-[15N,1H]-TROSY-iHNCA

Instrument

Bruker Avance - 500 MHz 1H{13C,15N}-triple-resonance three-axis PFG probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	RNase_T1	[U-95% 13C; U-95% 15N]	2 mM
2	D2O	[U-2H]	10 %
3	H2O	natural abundance	90 %

5 3D Cb-coupled [15N,1H]-TROSY-HNCA

Instrument

Bruker Avance - 800 MHz cryogenic triple-resonance z-gradient probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	RNase_T1	[U-95% 13C; U-95% 15N]	2 mM
2	D2O	[U-2H]	10 %
3	H2O	natural abundance	90 %

6 3D Ha-coupled [15N,1H]-TROSY-H(N)COCA

Instrument

Bruker Avance - 500 MHz 1H{13C,15N}-triple-resonance three-axis PFG probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	RNase_T1	[U-95% 13C; U-95% 15N]	2 mM
2	D2O	[U-2H]	10 %
3	H2O	natural abundance	90 %

7 3D Cb-coupled [15N,1H]-TROSY-H(N)COCA

Instrument

Bruker Avance - 500 MHz 1H{13C,15N}-triple-resonance three-axis PFG probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	RNase_T1	[U-95% 13C; U-95% 15N]	2 mM
2	D2O	[U-2H]	10 %
3	H2O	natural abundance	90 %

8 2D Cb-coupled [13C,1H]-HSQC

Instrument

Bruker Avance - 950 MHz cryogenic triple-resonance z-gradient probe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
4	RNase_T1	[U-95% 13C; U-95% 15N]		2 mM
5	D2O	[U-2H]		100 %

9 2D CO-coupled ct-[13C,1H]-HSQC

Instrument

Bruker Avance - 600 MHz room-temperature and cryogenic triple-resonance z-gradient probes

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
4	RNase_T1	[U-95% 13C; U-95% 15N]		2 mM
5	D2O	[U-2H]		100 %

10 3D CO-coupled ct-[15N,1H]-TROSY-HNCA

Instrument

Bruker Avance - 600 MHz room-temperature and cryogenic triple-resonance z-gradient probes

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	RNase_T1	[U-95% 13C; U-95% 15N]	2 mM
2	D2O	[U-2H]	10 %
3	H2O	natural abundance	90 %

11 3D CO-coupled (H)CANNH

Instrument

Bruker Avance - 900 MHz cryogenic triple-resonance z-gradient probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	RNase_T1	[U-95% 13C; U-95% 15N]	2 mM
2	D2O	[U-2H]	10 %
3	H2O	natural abundance	90 %

Keywords data mining, statistics, torsion angles, two-bond coupling, 2JHACO measurement, 2JCOHN measurement, 2JN_CB measurement, 2JCOCA measurement;, 2JCOHN measurement;, 2JN_CO measurement;, TROSY method, 2JCAN_ measurement, 2JHNCA measurement, IPAP method, 2JN_HA measurement, 2JCOCA measurement

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Found 1 Document (0.72 seconds)

BMRB: 16580

Related entities 1. Ribonuclease T1, : 1 : 25 : 11 : 27 : 34 entities Detail

Experiments performed 11 experiments Detail

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