BMRBj - BMREntryMaster

1LY7

2J coupling constants in human Frataxin C-terminal domain (90-210)

Authors: Schmidt, J.M., Lohr, F.
Assembly: Frataxin
Entity: 1. Frataxin (polymer, Thiol state: not present), 121 monomers, 13491.81 Da Detail
MDETTYERLA EETLDSLAEF FEDLADKPYT FEDYDVSFGS GVLTVKLGGD LGTYVINKQT PNKQIWLSSP SSGPKRYDWT GKNWVYSHDG VSLHELLAAE LTKALKTKLD LSSLAYSGKD A
Formula weight: 13491.81 Da
Source organism: Homo sapiens
Exptl. method: solution NMR
Data set: coupling_constants
Coupling constant: 223 J values in 2 lists
Field strength (¹H) 900 MHz, Pressure 1 atm, Temperature 298 K, pH 8.0 Detail
Pressure 1 atm, Temperature 298 K, pH 8.0
Experiment name 2D IPAP-type HN(CO-a/b-NCa-J)-TROSY
List #1 2JCaN_, Field strength (¹H) 900 MHz
List #2 2JHNCa, Field strength (¹H) 900 MHz
Release date: 2010-02-07
Citation 1: Correlation of (2)J couplings with protein secondary structure
Schmidt, J.M., Hua, Y., Lohr, F.
Proteins (2010), 78, 1544-1562, PubMed 20131375 , DOI 10.1002/prot.22672 , Abstract
Citation 2: Transverse relaxation optimised spin-state selective NMR experiments for measurement of residual dipolar couplings
Permi, P., Annila, A.
J. Biomol. NMR (2000), 16, 221-227, PubMed 10805128 , Abstract

#	Name	Isotope labeling	Concentration
1	Frataxin	[U-95% 13C; U-95% 15N]	1.7 mM
2	TRIS	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	D2O	[U-2H]	5 %
5	H2O	natural abundance	95 %

Keywords data mining, statistics, torsion angles, two-bond coupling, 2JCAN_ measurement, 2JHNCA measurement, IPAP method